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Agilent offers the largest, most modern liquid chromatography (LC) column portfolios for small molecule analysis, with columns suitable for nearly all separation modes and applications.


Whether you are performing conventional or ultra-fast chromatography, separating biomolecules, or analyzing complex compounds, the industry’s highest-performing compounds deliver fast, reproducible results – all engineered with unparalleled quality and reliability.

Catalog No. Product Net Price
AGIL5190-2453 Agilent Bio WCX, NP10, 4.6 x 250 mm. A weak cation exchange HPLC column with nonporous particles. Particle size is 10 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1559 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 250 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 10 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 275 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2454 Agilent Bio WCX, NP10, 4 x 10 mm, guard. A weak cation exchange HPLC guard column with nonporous particles. Particle size is 10 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 545 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 10 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 275 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2455 Agilent Bio WCX, NP10, 4.6 x 250 mm, PEEK. A weak cation exchange HPLC column with nonporous particles. Particle size is 10 μm. This column is PEEK-lined to couple with bio-compatible LC systems such as the 1290 Infinity II Bio LC System. 1748 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 250 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Hardware
  • PEEK
  • LC Platform
  • BioInert
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 10 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 275 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2456 Agilent Bio WCX, NP10, 4.6 x 50 mm, guard, PEEK. A weak cation exchange HPLC guard column with nonporous particles. Particle size is 10 μm. This column is PEEK-lined to couple with bio-compatible LC systems such as the 1290 Infinity II Bio LC System. 838 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 50 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Guard Column
  • Yes
  • Hardware
  • PEEK
  • LC Platform
  • BioInert
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 10 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 275 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2459 Agilent Bio WCX, NP10, 2.1 x 250 mm, PEEK. A weak cation exchange HPLC column with nonporous particles. Particle size is 10 μm. This column is PEEK-lined to couple with bio-compatible LC systems such as the 1290 Infinity II Bio LC System. 1929 
Add to Cart
  • Inner Diameter (ID)
  • 2.1 mm
  • Length
  • 250 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Hardware
  • PEEK
  • LC Platform
  • BioInert
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 10 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 275 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2460 Agilent Bio WCX, NP10, 2.1 x 50 mm, guard, PEEK. A weak cation exchange HPLC guard column with nonporous particles. Particle size is 10 μm. This column is PEEK-lined to couple with bio-compatible LC systems such as the 1290 Infinity II Bio LC System. 838 
Add to Cart
  • Inner Diameter (ID)
  • 2.1 mm
  • Length
  • 50 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Guard Column
  • Yes
  • Hardware
  • PEEK
  • LC Platform
  • BioInert
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 10 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 275 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2461 Agilent Bio SAX, NP1.7, 4.6 x 50 mm. A strong anion exchange HPLC column with nonporous particles. Particle size is 1.7 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1530 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 50 mm
  • Phase
  • SAX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SAX
  • Guard Column
  • No
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Monoclonal Antibodies (mABs) Proteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 1.7 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 689 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2462 Agilent Bio SAX, NP1.7, 4 x 10 mm, guard. A strong anion exchange HPLC guard column with nonporous particles. Particle size is 1.7 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 763 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • SAX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SAX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Monoclonal Antibodies (mABs) Proteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 1.7 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 689 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2463 Agilent Bio SAX, NP3, 4.5 x 50 mm, guard. A strong anion exchange HPLC guard column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1409 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 50 mm
  • Phase
  • SAX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SAX
  • Guard Column
  • No
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Monoclonal Antibodies (mABs) Proteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 3 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 551 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2464 Agilent Bio SAX, NP3, 4 x 10 mm, guard. A strong anion exchange HPLC guard column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 689 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • SAX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SAX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Monoclonal Antibodies (mABs) Proteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 3 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 551 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC

Overview

The small molecule column portfolio includes state-of-the-art superficially porous InfinityLab Poroshell 120 columns as well as traditional fully porous ZORBAX, Polaris, and Pursuit columns.

When you choose Agilent small molecule LC columns, you get more than just a dependable product. You also get unmatched technical support from the world’s largest chromatography supplier.


Agilent gel permeation chromatography (GPC) and size exclusion chromatography (SEC) columns and standards have been the top-selling in the polymer industry for over 40 years. GPC and SEC is used to determine the molecular weight and size distribution of macromolecules. SEC and GPC columns generate accurate, reliable data for critical polymer parameters, including dispersity (PDI), molecular weight mass average (Mw), number average (Mn), and z-average (Mz).


The importance of understanding the attributes of a biologic drug, and the processes used to create it, cannot be underestimated.  Agilent AdvanceBio LC columns are designed and produced to provide results you can be sure of when analyzing highly complex biotherapeutic molecules and monitoring their purity, potency, and other critical quality attributes.



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