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Agilent offers the largest, most modern liquid chromatography (LC) column portfolios for small molecule analysis, with columns suitable for nearly all separation modes and applications.


Whether you are performing conventional or ultra-fast chromatography, separating biomolecules, or analyzing complex compounds, the industry’s highest-performing compounds deliver fast, reproducible results – all engineered with unparalleled quality and reliability.

Catalog No. Product Net Price
AGIL5190-2441 Agilent Bio WCX, NP1.7, 4.6 x 50 mm. A weak cation exchange HPLC column with nonporous particles. Particle size is 1.7 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1530 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 50 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 1.7 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 689 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2442 Agilent Bio WCX, NP1.7, 4 x 10 mm, guard. A weak cation exchange HPLC guard column with nonporous particles. Particle size is 1.7 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 763 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 1.7 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 689 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2443 Agilent Bio WCX, NP3, 4.5 x 50 mm. A weak cation exchange HPLC column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1409 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 50 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 3 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 551 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2444 Agilent Bio WCX, NP3, 4 x 10 mm, guard. A weak cation exchange HPLC guard column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 689 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 3 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 551 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2445 Agilent Bio WCX, NP5, 4.6 x 250 mm. A weak cation exchange HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1910 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 250 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 413 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2446 Agilent Bio WCX, NP5, 4 x 10 mm, guard. A weak cation exchange HPLC guard column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 618 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 413 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2447 Agilent Bio WCX, NP5, 4.6 x 250 mm, PEEK. A weak cation exchange HPLC column with nonporous particles. Particle size is 5 μm. This column is PEEK-lined to couple with bio-compatible LC systems such as the 1290 Infinity II Bio LC System. 2077 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 250 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Hardware
  • PEEK
  • LC Platform
  • BioInert
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 413 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2448 Agilent Bio WCX, NP5, 4.6 x 50 mm, guard, PEEK. A weak cation exchange HPLC guard column with nonporous particles. Particle size is 5 μm. This column is PEEK-lined to couple with bio-compatible LC systems such as the 1290 Infinity II Bio LC System. 1147 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 50 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Guard Column
  • Yes
  • Hardware
  • PEEK
  • LC Platform
  • BioInert
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 413 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2451 Agilent Bio WCX, NP5, 2.1 x 250 mm, PEEK. A weak cation exchange HPLC column with nonporous particles. Particle size is 5 μm. This column is PEEK-lined to couple with bio-compatible LC systems such as the 1290 Infinity II Bio LC System. 2220 
Add to Cart
  • Inner Diameter (ID)
  • 2.1 mm
  • Length
  • 250 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Hardware
  • PEEK
  • LC Platform
  • BioInert
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 413 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2452 Agilent Bio WCX, NP5, 2.1 x 50 mm, guard, PEEK. A weak cation exchange HPLC guard column with nonporous particles. Particle size is 5 μm. This column is PEEK-lined to couple with bio-compatible LC systems such as the 1290 Infinity II Bio LC System. 1092 
Add to Cart
  • Inner Diameter (ID)
  • 2.1 mm
  • Length
  • 50 mm
  • Phase
  • WCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio WCX
  • Guard Column
  • Yes
  • Hardware
  • PEEK
  • LC Platform
  • BioInert
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 413 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC

Overview

The small molecule column portfolio includes state-of-the-art superficially porous InfinityLab Poroshell 120 columns as well as traditional fully porous ZORBAX, Polaris, and Pursuit columns.

When you choose Agilent small molecule LC columns, you get more than just a dependable product. You also get unmatched technical support from the world’s largest chromatography supplier.


Agilent gel permeation chromatography (GPC) and size exclusion chromatography (SEC) columns and standards have been the top-selling in the polymer industry for over 40 years. GPC and SEC is used to determine the molecular weight and size distribution of macromolecules. SEC and GPC columns generate accurate, reliable data for critical polymer parameters, including dispersity (PDI), molecular weight mass average (Mw), number average (Mn), and z-average (Mz).


The importance of understanding the attributes of a biologic drug, and the processes used to create it, cannot be underestimated.  Agilent AdvanceBio LC columns are designed and produced to provide results you can be sure of when analyzing highly complex biotherapeutic molecules and monitoring their purity, potency, and other critical quality attributes.



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