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Agilent offers the largest, most modern liquid chromatography (LC) column portfolios for small molecule analysis, with columns suitable for nearly all separation modes and applications.


Whether you are performing conventional or ultra-fast chromatography, separating biomolecules, or analyzing complex compounds, the industry’s highest-performing compounds deliver fast, reproducible results – all engineered with unparalleled quality and reliability.

Catalog No. Product Net Price
PL1580-1250 AdvanceBio SEC 120 Å column, 1.9 µm, 4.6 x 30 mm. to protect and extend the lifetime of a longer analytical SEC column. 709 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 30 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115709
  • Brand
  • AdvanceBio SEC
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 1000 Da
  • Particle Size
  • 1.9 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 120 Å
  • Pressure Rating
  • 620 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer/0.02% Sodium azide
  • Technique
  • LC
PL1580-3201 AdvanceBio SEC 200 Å, 4.6 x 150 mm, 1.9 µm, LC column. A size exclusion column designed to improve resolution and provide faster separation of monoclonal antibody aggregates and fragments by UHPLC. The resolving range is 2-700 kDa. 2339 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 150 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115709
  • Brand
  • AdvanceBio SEC
  • Guard Column
  • No
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C (20 to 40 °C recommended)
  • Molecular Weight Lower Limit
  • 2000 Da
  • Particle Size
  • 1.9 µm
  • pH Range
  • 2-8.5
  • Pore Size
  • 200 Å
  • Pressure Rating
  • 620 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer/0.02% Sodium azide
PL1580-5201 AdvanceBio SEC 200 Å, 4.6 x 300 mm, 1.9 µm, LC column. A size exclusion column designed to improve resolution and provide faster separation of monoclonal antibody aggregates and fragments by UHPLC. The resolving range is 2-700 kDa. 3325 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 300 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115709
  • Brand
  • AdvanceBio SEC
  • Guard Column
  • No
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C (20 to 40 °C recommended)
  • Molecular Weight Lower Limit
  • 2000 Da
  • Particle Size
  • 1.9 µm
  • pH Range
  • 2-8.5
  • Pore Size
  • 200 Å
  • Pressure Rating
  • 620 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer/0.02% Sodium azide
PL1580-5250 AdvanceBio SEC 120 Å column, 1.9 µm, 4.6 x 300 mm. A size exclusion column designed to improve resolution and provide faster separation of protein and peptide aggregates and fragments by UHPLC. Resolving range is 1-80 kDa. 3325 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 300 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115709
  • Brand
  • AdvanceBio SEC
  • Guard Column
  • No
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 1000 Da
  • Particle Size
  • 1.9 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 120 Å
  • Pressure Rating
  • 620 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer/0.02% Sodium azide
  • Technique
  • LC
AGIL5190-2421 Agilent Bio SCX, NP1.7, 4.6 x 50 mm. A strong cation exchange HPLC column with nonporous particles. Particle size is 1.7 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1530 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 50 mm
  • Phase
  • SCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SCX
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 1.7 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 689 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2422 Agilent Bio SCX, NP1.7, 4 x 10 mm, guard. A strong cation exchange HPLC guard column with nonporous particles. Particle size is 1.7 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 763 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • SCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SCX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 1.7 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 689 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2423 Agilent Bio SCX, NP3, 4.6 x 50 mm. A strong cation exchange HPLC column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1409 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 50 mm
  • Phase
  • SCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SCX
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 3 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 551 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2424 Agilent Bio SCX, NP3, 4 x 10 mm, guard. A strong cation exchange HPLC guard column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 689 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • SCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SCX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 3 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 551 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2425 Agilent Bio SCX, NP5, 4.6 x 250 mm. A strong cation exchange HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 1910 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 250 mm
  • Phase
  • SCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SCX
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 413 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2426 Agilent Bio SCX, NP5, 4 x 10 mm, guard. A strong cation exchange HPLC guard column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations. 618 
Add to Cart
  • Inner Diameter (ID)
  • 4 mm
  • Length
  • 10 mm
  • Phase
  • SCX
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SCX
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Part Number Application
  • Charge Variant Analysis PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Non-porous
  • pH Range
  • 2-12
  • Pressure Rating
  • 413 bar
  • Separation Mode
  • Ion Exchange (IEX)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC

Overview

The small molecule column portfolio includes state-of-the-art superficially porous InfinityLab Poroshell 120 columns as well as traditional fully porous ZORBAX, Polaris, and Pursuit columns.

When you choose Agilent small molecule LC columns, you get more than just a dependable product. You also get unmatched technical support from the world’s largest chromatography supplier.


Agilent gel permeation chromatography (GPC) and size exclusion chromatography (SEC) columns and standards have been the top-selling in the polymer industry for over 40 years. GPC and SEC is used to determine the molecular weight and size distribution of macromolecules. SEC and GPC columns generate accurate, reliable data for critical polymer parameters, including dispersity (PDI), molecular weight mass average (Mw), number average (Mn), and z-average (Mz).


The importance of understanding the attributes of a biologic drug, and the processes used to create it, cannot be underestimated.  Agilent AdvanceBio LC columns are designed and produced to provide results you can be sure of when analyzing highly complex biotherapeutic molecules and monitoring their purity, potency, and other critical quality attributes.



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