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Thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC), also called planar chromatography are based on a multistage distribution process involving

* Suitable adsorbents (the stationary phase) coated as a thin layer onto a suitable support / backing (e.g., glass plate, polyester or aluminum sheet)

* Solvents or solvent mixtures (the mobile phase or eluent)

* Sample molecules (analytes)

Today TLC has gained increasing importance as an analytical separation technique, which is probably due to effects of instrumentation and automation. At the same time the applicability of thin layer chromatography was enhanced by development of new adsorbents and backings. MACHEREY-NAGEL offers a versatile range of ready-to-use layers, which are the result of 50 years of continuous research and development.


Catalog No. Product Net Price
MN814018 TLC developing chamber, up to 2 plates/sheets simultaneous, format max. 10x10 cm Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT
MN814019 TLC developing chamber, up to 5 plates/sheets simultaneous, format max. 20x20 cm Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT
MN814103 Filter paper sheets MN 713, 15x21 cm Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT
MN814022 TLC glass capillaries, 1 µL Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT
MN818171 TLC aluminum sheets, modified silica gel layer, ALUGRAM RP-2 UV254, 20x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC)
  • Brand
  • ALUGRAM
  • Phase
  • RP-2 UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Aluminum sheets coated with modified silica gel
  • Surface chemistry
  • "Silica gel with "silanized silica" = dimethyl (RP2, C2) modification"
  • Recommended application(s)
  • Active plant constituents, Reversed phase (RP) and Normal phase (NP), Steroids
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 5–17 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT
MN818182 HPTLC/TLC aluminum sheets, mod. silica gel, ALUGRAM Nano-SIL NH2 UV254, 4x8 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • ALUGRAM
  • Phase
  • Nano-SIL NH2 UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Aluminum sheets coated with modified silica gel
  • Surface chemistry
  • Silica gel with Amino (NH2) modification
  • Recommended application(s)
  • Nucleotides, Pesticides, Phenols, Purine derivatives, Reversed phase (RP) and Normal phase (NP), Steroids, Sugars, Vitamins, Xanthines
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN818184 HPTLC/TLC aluminum sheets, mod. silica gel, ALUGRAM Nano-SIL CN UV254, 4x8 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • ALUGRAM
  • Phase
  • Nano-SIL CN UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Aluminum sheets coated with modified silica gel
  • Surface chemistry
  • Silica gel with Cyano (CN), nitrile modification
  • Recommended application(s)
  • Phenols, Preservatives, Reversed phase (RP) and Normal phase (NP), Steroid hormones
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN818144 HPTLC/TLC aluminum sheets, modified silica gel, ALUGRAM RP-18W UV254, 4x8 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • ALUGRAM
  • Phase
  • RP-18 W UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Aluminum sheets coated with modified silica gel
  • Surface chemistry
  • Silica gel with octadecyl (C18, ODS, RP18) modification
  • Recommended application(s)
  • Aminophenols, Barbiturates, HPTLC, Nucleobases, Phthalates, Plasticizers, Polycyclic aromatic hydrocarbons (PAH), Preservatives, Reversed phase, Steroids, Tetracyclines, Wettable with water
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT
MN818145 HPTLC/TLC aluminum sheets, modified silica gel, ALUGRAM RP-18W UV254, 5x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • ALUGRAM
  • Phase
  • RP-18 W UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Aluminum sheets coated with modified silica gel
  • Surface chemistry
  • Silica gel with octadecyl (C18, ODS, RP18) modification
  • Recommended application(s)
  • Aminophenols, Barbiturates, HPTLC, Nucleobases, Phthalates, Plasticizers, Polycyclic aromatic hydrocarbons (PAH), Preservatives, Reversed phase, Steroids, Tetracyclines, Wettable with water
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT
MN818146 HPTLC/TLC aluminum sheets, modified silica gel, ALUGRAM RP-18W UV254, 20x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • ALUGRAM
  • Phase
  • RP-18 W UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Aluminum sheets coated with modified silica gel
  • Surface chemistry
  • Silica gel with octadecyl (C18, ODS, RP18) modification
  • Recommended application(s)
  • Aminophenols, Barbiturates, HPTLC, Nucleobases, Phthalates, Plasticizers, Polycyclic aromatic hydrocarbons (PAH), Preservatives, Reversed phase, Steroids, Tetracyclines, Wettable with water
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT

Overview

The success of thin layer chromatography as a highly efficient microanalytical separation method is based on a large number of advantageous properties:


High sample throughput in a short time

Suitable for screening tests

Pilot procedure for HPLC and Flash chromatography

After separation the analytical information can be stored for a longer period of time (the TLC ready-to-use layer acts as storage medium for data)

Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later date

Rapid and cost-efficient optimization of the separation due to easy change of mobile and stationary phase



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