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Thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC), also called planar chromatography are based on a multistage distribution process involving

* Suitable adsorbents (the stationary phase) coated as a thin layer onto a suitable support / backing (e.g., glass plate, polyester or aluminum sheet)

* Solvents or solvent mixtures (the mobile phase or eluent)

* Sample molecules (analytes)

Today TLC has gained increasing importance as an analytical separation technique, which is probably due to effects of instrumentation and automation. At the same time the applicability of thin layer chromatography was enhanced by development of new adsorbents and backings. MACHEREY-NAGEL offers a versatile range of ready-to-use layers, which are the result of 50 years of continuous research and development.


Catalog No. Product Net Price
MN811058 HPTLC/TLC glass plates, silica layer with chiral selector, CHIRALPLATE, 20x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Phase
  • CHIRALPLATE
  • Mode
  • Reversed phase (RP) with ligand exchange
  • Base material
  • Glass plates coated with impregnated silica gel
  • Surface chemistry
  • Silica gel impregnated with Cu(II) ions and a chiral selector (proline derivative)
  • Recommended application(s)
  • Amino acids, Dipeptides, Enantiomers, Lactones, N-formylamino acids, N-methylamino acids, Thiazolidine derivatives, α-alkylamino acids, α-hydroxycarboxylic acids
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN811059 HPTLC/TLC glass plates, silica layer with chiral selector, CHIRALPLATE, 10x10 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Phase
  • CHIRALPLATE
  • Mode
  • Reversed phase (RP) with ligand exchange
  • Base material
  • Glass plates coated with impregnated silica gel
  • Surface chemistry
  • Silica gel impregnated with Cu(II) ions and a chiral selector (proline derivative)
  • Recommended application(s)
  • Amino acids, Dipeptides, Enantiomers, Lactones, N-formylamino acids, N-methylamino acids, Thiazolidine derivatives, α-alkylamino acids, α-hydroxycarboxylic acids
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN814301 TLC reference solution, cholesterol Special offer 
Add to Cart
  • Method
  • TLC Micro-Set, accessory
  • Storage temperature
  • 4−8 °C
MN814030 Chromatography paper MN 260, 7.5x17 cm Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT
MN814202 TLC test mixture for Micro-Set F1, individual, amino acids Special offer 
Add to Cart
  • Method
  • TLC Micro-Set, accessory
  • Storage temperature
  • 4−8 °C
MN814205 TLC test mixture for Micro-Set F1, individual, heavy metal cations Special offer 
Add to Cart
  • Method
  • TLC Micro-Set, accessory
  • Storage temperature
  • 4−8 °C
MN814011 TLC test mixture for Micro-Set A, individual, fat-soluble / lipophilic dyes Special offer 
Add to Cart
  • Method
  • TLC Micro-Set, accessory
  • Storage temperature
  • 4−8 °C
MN814012 TLC test mixture for Micro-Set A, individual, anthraquinone dyes Special offer 
Add to Cart
  • Method
  • TLC Micro-Set, accessory
  • Storage temperature
  • 4−8 °C
MN814013 TLC test mixture for Micro-Set A, individual, food dyes Special offer 
Add to Cart
  • Method
  • TLC Micro-Set, accessory
  • Storage temperature
  • 4−8 °C
MN814028 TLC polyester sheets, POLYGRAM mix Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Brand
  • POLYGRAM
  • Storage temperature
  • RT

Overview

The success of thin layer chromatography as a highly efficient microanalytical separation method is based on a large number of advantageous properties:


High sample throughput in a short time

Suitable for screening tests

Pilot procedure for HPLC and Flash chromatography

After separation the analytical information can be stored for a longer period of time (the TLC ready-to-use layer acts as storage medium for data)

Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later date

Rapid and cost-efficient optimization of the separation due to easy change of mobile and stationary phase



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