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Thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC), also called planar chromatography are based on a multistage distribution process involving

* Suitable adsorbents (the stationary phase) coated as a thin layer onto a suitable support / backing (e.g., glass plate, polyester or aluminum sheet)

* Solvents or solvent mixtures (the mobile phase or eluent)

* Sample molecules (analytes)

Today TLC has gained increasing importance as an analytical separation technique, which is probably due to effects of instrumentation and automation. At the same time the applicability of thin layer chromatography was enhanced by development of new adsorbents and backings. MACHEREY-NAGEL offers a versatile range of ready-to-use layers, which are the result of 50 years of continuous research and development.


Catalog No. Product Net Price
MN811213 HPTLC/TLC glass plates, silica gel layer, Nano-SIL HD, 10x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • Nano-SIL HD
  • Phase
  • Nano-SIL HD
  • Mode
  • Normal phase (NP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Unmodified silica gel (SiOH)
  • Recommended application(s)
  • Aflatoxins, Caffeine in drinks, Dyes, Flavonoids, Insecticides, Normal phase (NP), Peppermint oil, Pesticides, Steroids, Sulfonamide, UV filter in sunscreen, Vitamins
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN811221 HPTLC/TLC glass plates, silica gel layer, Nano-SIL HD UV254, 5x5 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • Nano-SIL HD
  • Phase
  • Nano-SIL HD UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Unmodified silica gel (SiOH)
  • Recommended application(s)
  • Aflatoxins, Caffeine in drinks, Dyes, Flavonoids, Insecticides, Normal phase (NP), Peppermint oil, Pesticides, Steroids, Sulfonamide, UV filter in sunscreen, Vitamins
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN811222 HPTLC/TLC glass plates, silica gel layer, Nano-SIL HD UV254, 10x10 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • Nano-SIL HD
  • Phase
  • Nano-SIL HD UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Unmodified silica gel (SiOH)
  • Recommended application(s)
  • Aflatoxins, Caffeine in drinks, Dyes, Flavonoids, Insecticides, Normal phase (NP), Peppermint oil, Pesticides, Steroids, Sulfonamide, UV filter in sunscreen, Vitamins
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN811223 HPTLC/TLC glass plates, silica gel layer, Nano-SIL HD UV254, 10x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • Nano-SIL HD
  • Phase
  • Nano-SIL HD UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Unmodified silica gel (SiOH)
  • Recommended application(s)
  • Aflatoxins, Caffeine in drinks, Dyes, Flavonoids, Insecticides, Normal phase (NP), Peppermint oil, Pesticides, Steroids, Sulfonamide, UV filter in sunscreen, Vitamins
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN814403 TLC visualization (staining) reagent, iron(III) chloride solution, 100 mL Special offer 
Add to Cart
  • Method
  • TLC, visualization
  • Storage temperature
  • 4−8 °C
MN814101 Laboratory reagent sprayer, glass with rubber bulb Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT
MN814021 TLC developing chamber (jar shaped) for small TLC formats Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT
MN814024 Measuring cylinder, glass, 10 mL Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT
MN814200 TLC Micro-Set F1, advanced set for science education Special offer 
Add to Cart
  • Method
  • TLC Micro-Set
  • Storage temperature
  • 4−8 °C
MN814104 Plastic syringe, 1 mL with graduation Special offer 
Add to Cart
  • Method
  • TLC, accessory
  • Storage temperature
  • RT

Overview

The success of thin layer chromatography as a highly efficient microanalytical separation method is based on a large number of advantageous properties:


High sample throughput in a short time

Suitable for screening tests

Pilot procedure for HPLC and Flash chromatography

After separation the analytical information can be stored for a longer period of time (the TLC ready-to-use layer acts as storage medium for data)

Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later date

Rapid and cost-efficient optimization of the separation due to easy change of mobile and stationary phase



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