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Thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC), also called planar chromatography are based on a multistage distribution process involving

* Suitable adsorbents (the stationary phase) coated as a thin layer onto a suitable support / backing (e.g., glass plate, polyester or aluminum sheet)

* Solvents or solvent mixtures (the mobile phase or eluent)

* Sample molecules (analytes)

Today TLC has gained increasing importance as an analytical separation technique, which is probably due to effects of instrumentation and automation. At the same time the applicability of thin layer chromatography was enhanced by development of new adsorbents and backings. MACHEREY-NAGEL offers a versatile range of ready-to-use layers, which are the result of 50 years of continuous research and development.


Catalog No. Product Net Price
MN814203 TLC visualization (staining) reagent, ninhydrin, 100 mL Special offer 
Add to Cart
  • Method
  • TLC, visualization
  • Storage temperature
  • 4−8 °C
MN814923 TLC visualization (staining) reagent, rhodamin B, 100 mL Special offer 
Add to Cart
  • Method
  • TLC, visualization
  • Storage temperature
  • 4−8 °C
MN814206 TLC visualization (staining) reagent, rubeanic acid, 100 mL Special offer 
Add to Cart
  • Method
  • TLC, visualization
  • Storage temperature
  • 4−8 °C
MN811071 HPTLC glass plates RP-18 W UV254, C18-modified silica layer, 20x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • RP-18 W
  • Phase
  • RP-18 W UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Silica gel with octadecyl (C18, ODS, RP18) modification
  • Recommended application(s)
  • Aminophenols, Barbiturates, HPTLC, Nucleobases, Phthalates, Plasticizers, Polycyclic aromatic hydrocarbons (PAH), Preservatives, Reversed phase, Steroids, Tetracyclines, Wettable with water
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT
MN811072 HPTLC glass plates RP-18 W UV254, C18-modified silica layer, 10x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • RP-18 W
  • Phase
  • RP-18 W UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Silica gel with octadecyl (C18, ODS, RP18) modification
  • Recommended application(s)
  • Aminophenols, Barbiturates, HPTLC, Nucleobases, Phthalates, Plasticizers, Polycyclic aromatic hydrocarbons (PAH), Preservatives, Reversed phase, Steroids, Tetracyclines, Wettable with water
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT
MN811073 HPTLC glass plates RP-18 W UV254, C18-modified silica layer, 5x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • RP-18 W
  • Phase
  • RP-18 W UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Silica gel with octadecyl (C18, ODS, RP18) modification
  • Recommended application(s)
  • Aminophenols, Barbiturates, HPTLC, Nucleobases, Phthalates, Plasticizers, Polycyclic aromatic hydrocarbons (PAH), Preservatives, Reversed phase, Steroids, Tetracyclines, Wettable with water
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT
MN811074 TLC glass plates RP-18 W UV254, C18-modified silica layer, 20x20 cm, preparative Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC), preparative (PLC)
  • Brand
  • RP-18 W
  • Phase
  • RP-18 W UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Silica gel with octadecyl (C18, ODS, RP18) modification
  • Recommended application(s)
  • Aminophenols, Barbiturates, Nucleobases, Phthalates, Plasticizers, Polycyclic aromatic hydrocarbons (PAH), Preservatives, RP applications (preparative), Steroids, Tetracyclines, Wettable with water
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 5–17 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT
MN811075 HPTLC glass plates RP-18 W UV254, C18-modified silica layer, 10x10 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (HPTLC/TLC)
  • Brand
  • RP-18 W
  • Phase
  • RP-18 W UV254
  • Mode
  • Reversed phase (RP)
  • Base material
  • Glass plates coated with silica gel
  • Surface chemistry
  • Silica gel with octadecyl (C18, ODS, RP18) modification
  • Recommended application(s)
  • Aminophenols, Barbiturates, HPTLC, Nucleobases, Phthalates, Plasticizers, Polycyclic aromatic hydrocarbons (PAH), Preservatives, Reversed phase, Steroids, Tetracyclines, Wettable with water
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–10 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–10.0
  • Storage temperature
  • RT
MN816720.01 Fluorescent indicators for TLC, UV366, blue fluorescence Special offer 
Add to Cart
  • Method
  • Thin layer chromatography (TLC), adsorbents
  • Mode
  • Not available or not applicable
  • Base material
  • Inorganic fluorescent pigment
  • Surface chemistry
  • Not available or not applicable
  • Recommended application(s)
  • TLC fluorescent indicator
  • Particle type
  • Not available or not applicable
  • Particle shape
  • Irregular
  • Specific surface according to BET
  • Not available or not applicable
  • pH stability
  • Not available or not applicable
  • Storage temperature
  • RT
MN816710.01 Fluorescent indicators for TLC, UV254, green fluorescence Special offer 
Add to Cart
  • Method
  • Thin layer chromatography (TLC), adsorbents
  • Mode
  • Not available or not applicable
  • Base material
  • Manganese activated zinc silicate
  • Surface chemistry
  • Not available or not applicable
  • Recommended application(s)
  • TLC fluorescent indicator
  • Particle type
  • Not available or not applicable
  • Particle shape
  • Irregular
  • Specific surface according to BET
  • Not available or not applicable
  • pH stability
  • Not available or not applicable
  • Storage temperature
  • RT

Overview

The success of thin layer chromatography as a highly efficient microanalytical separation method is based on a large number of advantageous properties:


High sample throughput in a short time

Suitable for screening tests

Pilot procedure for HPLC and Flash chromatography

After separation the analytical information can be stored for a longer period of time (the TLC ready-to-use layer acts as storage medium for data)

Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later date

Rapid and cost-efficient optimization of the separation due to easy change of mobile and stationary phase



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