MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN711992.10	LC packing material (adsorbents, bulk), silica gel, POLYGOSIL 1000-7 C18	Special offer	Add to Cart
Hazardous material No Method LC, packing material (adsorbent, bulk) Brand POLYGOSIL Phase POLYGOSIL 1000-7 C18 Mode Normal phase (NP) Base material Silica gel Surface chemistry Octadecyl (C18, ODS) modification, Octadecyl phase (C18, ODS) modification Endcapped Yes Selling unit 10 g, 100 g Recommended application(s) LC packing material for analytical applications Particle type Fully porous particles (FPP) Particle size 7 µm Pore size 1000 Å Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT
	MN732712	CHROMABOND Flash RS 25 C18 ec cartridges, 15–40 µm, 26 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 17.3 cm Column inner diameter 21.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 26 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 25–70 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 1x CHROMABOND Flash RS cartridge Storage temperature RT
	MN732713	CHROMABOND Flash RS 40 C18 ec cartridges, 15–40 µm, 43 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 17.6 cm Column inner diameter 26.7 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 43 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 35–80 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 1x CHROMABOND Flash RS cartridge Storage temperature RT
	MN732714	CHROMABOND Flash RS 80 C18 ec cartridges, 15–40 µm, 86 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 24.9 cm Column inner diameter 30.9 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 86 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 45–105 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 1x CHROMABOND Flash RS cartridge Storage temperature RT
	MN732715	CHROMABOND Flash RS 120 C18 ec cartridges, 15–40 µm, 130 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 26.2 cm Column inner diameter 37.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 130 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 55–155 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 1x CHROMABOND Flash RS cartridge Storage temperature RT
	MN732716	CHROMABOND Flash RS 200 C18 ec cartridges, 15–40 µm, 220 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 21.6 cm Column inner diameter 59.4 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 220 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 75–255 mL/min pH stability 2.0–8.0 Pressure stability (max) 11 bar Filter element material Polyethylene (PE) Scope of delivery 1x CHROMABOND Flash RS cartridge Storage temperature RT
	MN732717	CHROMABOND Flash RS 330 C18 ec cartridges, 15–40 µm, 360 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 28.0 cm Column inner diameter 59.8 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 360 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 75–255 mL/min pH stability 2.0–8.0 Pressure stability (max) 11 bar Filter element material Polyethylene (PE) Scope of delivery 1x CHROMABOND Flash RS cartridge Storage temperature RT
	MN732720	CHROMABOND Flash RS 4 C18 ec cartridges, 15–40 µm, 4.3 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 10.6 cm Column inner diameter 12.4 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 5 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 10–25 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 2x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732810	CHROMABOND Flash RS 4 C18 ec cartridges, 40–63 µm, 4.3 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 10.6 cm Column inner diameter 12.4 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 4.3 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 10–35 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 2x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732811	CHROMABOND Flash RS 15 C18 ec cartridges, 40–63 µm, 16.4 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Endcapped Hardware Column type Flash RS Column length 12.5 cm Column inner diameter 21.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 16.4 g Recommended application(s) Reversed phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 25–55 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 1x CHROMABOND Flash RS cartridge Storage temperature RT

<< 43 44 45 46 474849 50 51 52 53 >>

Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

Related Categories