+852-3069 6950
css@gugent.com.hk
About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents.
SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique).
SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.
| Catalog No. | Product | Net Price | |||
|---|---|---|---|---|---|
| MN738570.025M | SPE MULTI 96-well plate CHROMABOND HR-XCW, 96x 25 mg, 45 µm | Special offer | |||
|
|||||
| MN738071.100M | SPE MULTI 96-well plate CHROMABOND SiOH, 96x 100 mg, 45 µm | Special offer | |||
|
|||||
| MN738131.150M | SPE MULTI 96-well plate CHROMABOND XTR, LLE/SLE, 96x 150 mg | Special offer | |||
|
|||||
| MN730106 | CHROMABOND stainless steel connectors for vacuum manifolds | Special offer | |||
|
|||||
| MN730177N | Gasket for lid of CHROMABOND vacuum manifold with 12 positions (REF 730150N) | Special offer | |||
|
|||||
| MN730178N | Gasket for lid of CHROMABOND vacuum manifold with 24 positions (REF 730151N) | Special offer | |||
|
|||||
| MN727401 | Flash chromatography column, glass,complete with adaptor,400 mm L,20 mm ID,empty | Special offer | |||
|
|||||
| MN727407 | Flash chromatography column, glass,complete with adaptor,450 mm L,40 mm ID,empty | Special offer | |||
|
|||||
| MN730191 | Glass fiber filter elements for CHROMABOND SPE glass empty columns, 3 mL | Special offer | |||
|
|||||
| MN730192 | Glass fiber filter elements for CHROMABOND SPE glass empty columns, 6 mL | Special offer | |||
|
|||||
Main steps of the SPE procedure
1) Conditioning
Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).
2) Sample application (adsorption)
Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.
3) Washing
Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.
4) Elution
Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).
Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.
Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks
Brochure-CHROMABOND-QuEChERS-EN
User manual CHROMABOND SPE vacuum manifolds
