MACHEREY-NAGEL - LC Columns - Chromatography - Lab supplies

Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork. * NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses. * NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications. * Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No.		Product	Net Price
	MN760410.20	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 50x2 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 50 mm Column inner diameter 2 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 0.15 mL
	MN760410.30	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 50x3 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 50 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 0.35 mL
	MN760410.40	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 50x4 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 50 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 0.62 mL
	MN760410.46	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 50x4.6 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 50 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 0.83 mL
	MN760412.20	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 125x2 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 125 mm Column inner diameter 2 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 0.37 mL
	MN760412.30	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 125x3 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 125 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 0.87 mL
	MN760412.40	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 125x4 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 125 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 1.56 mL
	MN760412.46	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 125x4.6 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 125 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 2.06 mL
	MN760413.20	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 150x2 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 150 mm Column inner diameter 2 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 0.45 mL
	MN760413.30	EC HPLC column (analytical), NUCLEODUR C18 Isis, 5 µm, 150x3 mm	Special offer	Add to Cart
Recommended application(s) (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR C18 Isis USP listing L1 Surface chemistry High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 150 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 1.0–10.0 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 1.05 mL

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Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.

Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.

Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.

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