MACHEREY-NAGEL - LC Columns - Chromatography - Lab supplies

Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork. * NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses. * NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications. * Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No.		Product	Net Price
	MN719414	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 10000-10, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 10 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719469	VA HPLC column (analytical), NUCLEOGEL SAX, 8 µm, 50x4.6 mm	Special offer	Add to Cart
Recommended application(s) Anion exchange, Biological macromolecules, HPLC, Large proteins and oligonucleotides, Purification of peptides Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polymer-based Phase NUCLEOGEL SAX USP listing L23 Surface chemistry Polymer-based strongly basic anion exchanger -N+(CH3)3, gel matrix quaternized PEI Mode Ion exchange (IEX) Type VA HPLC column (analytical) Hardware Stainless steel Column length 50 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8 µm Eluent in HPLC column 0.1 mol/L Na₂SO₄ (sodium sulfate) + 0.02 % NaN₃ (sodium azide) Endcapped No Carbon content Not available or proprietary pH stability 1.0–14.0 Pore size 1000 Å Pressure stability (max) 200 bar Column volume 0.83 mL
	MN719533	VA HPLC column (analytical), NUCLEOGEL Anion I, 10 µm, 120x4.6 mm	Special offer	Add to Cart
Recommended application(s) Anions, Bromide, Chloride, Fluoride, HPLC, Ion chromatography, Nitrate, Nitrite, Phosphate, Sulfate Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polymer-based Phase NUCLEOGEL Anion I USP listing Not available or proprietary Surface chemistry Strongly basic polymer-based anion exchanger Mode Ion exchange (IEX), Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 120 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 10 µm Eluent in HPLC column 4 mmol/L salicylate buffer, pH 7.8 Endcapped No Carbon content Not available or proprietary pH stability 1.0–14.0 Specific surface according to BET Not available or proprietary
	MN719475	VA HPLC column (analytical), NUCLEOGEL SCX, 8 µm, 50x4.6 mm	Special offer	Add to Cart
Recommended application(s) Carbohydrates with high isoelectric point, Cation exchange, HPLC, Peptides, Proteins Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polymer-based Phase NUCLEOGEL SCX USP listing L22 Surface chemistry Polymer-based strongly acidic cation exchanger SO₃⁻, hydrophilic gel matrix Mode Ion exchange (IEX) Type VA HPLC column (analytical) Hardware Stainless steel Column length 50 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8 µm Eluent in HPLC column 0.1 mol/L Na₂SO₄ (sodium sulfate) + 0.02 % NaN₃ (sodium azide) Endcapped No Carbon content Not available or proprietary pH stability 1.0–13.0 Pore size 1000 Å Pressure stability (max) 200 bar Column volume 0.83 mL
	MN719570	VA HPLC column (analytical), NUCLEOGEL SUGAR 810 Ca, 8-10 µm, 300x7.8 mm	Special offer	Add to Cart
Recommended application(s) HPLC, Mono- and oligosaccharides, Sugar alcohols, Sugars Storage temperature 2−8 °C Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL SUGAR 810 Ca USP listing L19 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), Ca²⁺ form Mode Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.8 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8–10 µm Eluent in HPLC column Demineralized water Endcapped No Carbon content Not available or proprietary Pore size 100 Å Temperature stability (max) 90 °C Pressure stability (max) 70 bar Column volume 14.3 mL
	MN719571	CC HPLC guard column (analytical), NUCLEOGEL SUGAR 810 Ca, 8-10 µm, 30x4 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 2x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL SUGAR 810 Ca USP listing L19 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), Ca²⁺ form Mode Special application or diverse Type CC HPLC guard column (analytical) Hardware Stainless steel Column length 30 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8–10 µm Eluent in HPLC column Demineralized water Endcapped No Carbon content Not available or proprietary Pore size 100 Å Temperature stability (max) 90 °C Pressure stability (max) 70 bar
	MN719531	VA HPLC column (analytical), NUCLEOGEL SUGAR Ca, 8-10 µm, 300x6.5 mm	Special offer	Add to Cart
Recommended application(s) HPLC, Mono- and oligosaccharides, Sugar alcohols, Sugars Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL SUGAR Ca USP listing L19 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), Ca²⁺ form Mode Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 6.5 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8–10 µm Eluent in HPLC column Demineralized water + 0.02 % NaN₃ (sodium azide) Endcapped No Carbon content Not available or proprietary Pore size 100 Å Temperature stability (max) 90 °C Pressure stability (max) 70 bar Column volume 9.95 mL
	MN719574	VA HPLC column (analytical), NUCLEOGEL SUGAR 810 H, 8-10 µm, 300x7.8 mm	Special offer	Add to Cart
Recommended application(s) Alcohols, Organic acids, Sugars Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL SUGAR 810 H USP listing L17 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), H⁺ form Mode Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.8 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8–10 µm Eluent in HPLC column 5 mmol/L H₂SO₄ (sulfuric acid) Endcapped No Carbon content Not available or proprietary Pore size 100 Å Temperature stability (max) 90 °C Pressure stability (max) 70 bar Column volume 14.3 mL
	MN719532	VA HPLC column (analytical), NUCLEOGEL SUGAR Na, 8-10 µm, 300x7.8 mm	Special offer	Add to Cart
Recommended application(s) HPLC, Oligosaccharides, Starch hydrolyzates and food, Sugars Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL SUGAR Na USP listing L58 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), Na⁺ form Mode Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.8 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8–10 µm Eluent in HPLC column Demineralized water + 0.02 % NaN₃ (sodium azide) Endcapped No Carbon content Not available or proprietary Pore size 100 Å Temperature stability (max) 90 °C Pressure stability (max) 70 bar Column volume 14.3 mL
	MN719530	VA HPLC column (analytical), NUCLEOGEL SUGAR Pb, 10 µm, 300x7.8 mm	Special offer	Add to Cart
Recommended application(s) Food and biological samples, HPLC, Mono- and oligosaccharides, Sugar alcohols, Sugars Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL SUGAR Pb USP listing L34 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), Pb²⁺ form Mode Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.8 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 10 µm Eluent in HPLC column Demineralized water + 0.02 % NaN₃ (sodium azide) Endcapped No Carbon content Not available or proprietary Pore size 100 Å Temperature stability (max) 90 °C Pressure stability (max) 70 bar Column volume 14.3 mL

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Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.

Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.

Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.

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