MACHEREY-NAGEL - LC Columns - Chromatography - Lab supplies

Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork. * NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses. * NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications. * Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No.		Product	Net Price
	MN719501	VA HPLC column (analytical), NUCLEOGEL ION 300 OA, 10 µm, 300x7.8 mm	Special offer	Add to Cart
Recommended application(s) Alcohols, HPLC, Organic acids, Sugars Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL ION 300 OA USP listing L17 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), H⁺ form Mode Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.8 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 10 µm Eluent in HPLC column 5 mmol/L H₂SO₄ (sulfuric acid) Endcapped No Carbon content Not available or proprietary Pore size 300 Å Specific surface according to BET 100 m²/g Temperature stability (max) 90 °C Pressure stability (max) 150 bar
	MN719537	VA HPLC guard column (analytical), NUCLEOGEL ION 300 OA, 10 µm, 21x4 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 2x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL ION 300 OA USP listing L17 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), H⁺ form Mode Special application or diverse Type VA HPLC guard column (analytical) Hardware Stainless steel Column length 21 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 10 µm Eluent in HPLC column 5 mmol/L H₂SO₄ (sulfuric acid) Endcapped No Carbon content Not available or proprietary Pore size 300 Å Specific surface according to BET 100 m²/g Temperature stability (max) 90 °C Pressure stability (max) 150 bar
	MN719454	VA HPLC column (analytical), NUCLEOGEL RP, 5 µm, 150x4.6 mm	Special offer	Add to Cart
Recommended application(s) Higher background hydrophobicity compared to silica phases, Large biomolecules, Peptides, Proteins, Reversed phase, RP applications HPLC Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polystyrene resin cross-linked with divinylbenzene (PS/DVB) Phase NUCLEOGEL RP USP listing L21 Surface chemistry Polystyrene resin cross-linked with divinylbenzene (PS/DVB) Mode Reversed phase (RP) Type VA HPLC column (analytical) Hardware Stainless steel Column length 150 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped No Carbon content Not available or proprietary pH stability 1.0–13.0 Pore size 100 Å Temperature stability (max) 80 °C Pressure stability (max) 180 bar Column volume 2.5 mL
	MN719455	VA HPLC column (analytical), NUCLEOGEL RP, 5 µm, 250x4.6 mm	Special offer	Add to Cart
Recommended application(s) Higher background hydrophobicity compared to silica phases, Large biomolecules, Peptides, Proteins, Reversed phase, RP applications HPLC Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polystyrene resin cross-linked with divinylbenzene (PS/DVB) Phase NUCLEOGEL RP USP listing L21 Surface chemistry Polystyrene resin cross-linked with divinylbenzene (PS/DVB) Mode Reversed phase (RP) Type VA HPLC column (analytical) Hardware Stainless steel Column length 250 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped No Carbon content Not available or proprietary pH stability 1.0–13.0 Pore size 100 Å Temperature stability (max) 80 °C Pressure stability (max) 180 bar Column volume 4.15 mL
	MN719456	VA HPLC column (analytical), NUCLEOGEL RP, 8 µm, 150x4.6 mm	Special offer	Add to Cart
Recommended application(s) Higher background hydrophobicity compared to silica phases, Large biomolecules, Peptides, Proteins, Reversed phase, RP applications HPLC Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polystyrene resin cross-linked with divinylbenzene (PS/DVB) Phase NUCLEOGEL RP USP listing L21 Surface chemistry Polystyrene resin cross-linked with divinylbenzene (PS/DVB) Mode Reversed phase (RP) Type VA HPLC column (analytical) Hardware Stainless steel Column length 150 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8 µm Eluent in HPLC column Acetonitrile – water Endcapped No Carbon content Not available or proprietary pH stability 1.0–13.0 Pore size 100 Å Temperature stability (max) 80 °C Pressure stability (max) 180 bar Column volume 2.5 mL
	MN720431.40	EC HPLC column (analytical), NUCLEOSIL 100-5 C18 Nautilus, 5 µm, 250x4 mm	Special offer	Add to Cart
Recommended application(s) Amino acids, Basic pharmaceuticals, Especially for polar compounds, Organic acids, Pesticides, Phenols and nitrogen containing compounds, Reversed phase, RP applications HPLC, Water-soluble vitamins Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 C18 Nautilus USP listing L1, L60 Surface chemistry Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 250 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 16.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar Column volume 3.15 mL
	MN720431.46	EC HPLC column (analytical), NUCLEOSIL 100-5 C18 Nautilus, 5 µm, 250x4.6 mm	Special offer	Add to Cart
Recommended application(s) Amino acids, Basic pharmaceuticals, Especially for polar compounds, Organic acids, Pesticides, Phenols and nitrogen containing compounds, Reversed phase, RP applications HPLC, Water-soluble vitamins Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 C18 Nautilus USP listing L1, L60 Surface chemistry Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 250 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 16.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar Column volume 4.15 mL
	MN720432.46	EC HPLC column (analytical), NUCLEOSIL 100-5 C18 Nautilus, 5 µm, 150x4.6 mm	Special offer	Add to Cart
Recommended application(s) Amino acids, Basic pharmaceuticals, Especially for polar compounds, Organic acids, Pesticides, Phenols and nitrogen containing compounds, Reversed phase, RP applications HPLC, Water-soluble vitamins Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 C18 Nautilus USP listing L1, L60 Surface chemistry Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 150 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 16.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar Column volume 2.5 mL
	MN720471.46	EC HPLC column (analytical), NUCLEOSIL 100-3 C18 Nautilus, 3 µm, 150x4.6 mm	Special offer	Add to Cart
Recommended application(s) Amino acids, Basic pharmaceuticals, Especially for polar compounds, Organic acids, Pesticides, Phenols and nitrogen containing compounds, Reversed phase, RP applications HPLC, Water-soluble vitamins Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-3 C18 Nautilus USP listing L1, L60 Surface chemistry Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 150 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 3 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 16.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar Column volume 2.5 mL
	MN720472.40	EC HPLC column (analytical), NUCLEOSIL 100-3 C18 Nautilus, 3 µm, 125x4 mm	Special offer	Add to Cart
Recommended application(s) Amino acids, Basic pharmaceuticals, Especially for polar compounds, Organic acids, Pesticides, Phenols and nitrogen containing compounds, Reversed phase, RP applications HPLC, Water-soluble vitamins Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-3 C18 Nautilus USP listing L1, L60 Surface chemistry Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) Mode Reversed phase (RP) Type EC HPLC column (analytical) Hardware Stainless steel Column length 125 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 3 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 16.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar Column volume 1.56 mL

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Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.

Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.

Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.

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