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Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork.

* NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses.

* NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications.

* Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No. Product Net Price
MN760639.30 EC HPLC column (analytical), NUCLEODUR π², 3 µm, 250x3 mm Special offer 
Add to Cart
  • Recommended application(s)
  • Antibiotics, Especially aromatic and unsaturated compounds, Especially for polar compounds, Pharmaceuticals, Reversed phase, RP applications HPLC, Steroids
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR π²
  • USP listing
  • L11
  • Surface chemistry
  • Biphenylpropyl modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 250 mm
  • Column inner diameter
  • 3 mm
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 17.0 %
  • pH stability
  • 3.0–10.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
MN760639.40 EC HPLC column (analytical), NUCLEODUR π², 3 µm, 250x4 mm Special offer 
Add to Cart
  • Recommended application(s)
  • Antibiotics, Especially aromatic and unsaturated compounds, Especially for polar compounds, Pharmaceuticals, Reversed phase, RP applications HPLC, Steroids
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR π²
  • USP listing
  • L11
  • Surface chemistry
  • Biphenylpropyl modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 250 mm
  • Column inner diameter
  • 4 mm
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 17.0 %
  • pH stability
  • 3.0–10.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
MN760639.46 EC HPLC column (analytical), NUCLEODUR π², 3 µm, 250x4.6 mm Special offer 
Add to Cart
  • Recommended application(s)
  • Antibiotics, Especially aromatic and unsaturated compounds, Especially for polar compounds, Pharmaceuticals, Reversed phase, RP applications HPLC, Steroids
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR π²
  • USP listing
  • L11
  • Surface chemistry
  • Biphenylpropyl modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 250 mm
  • Column inner diameter
  • 4.6 mm
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 17.0 %
  • pH stability
  • 3.0–10.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
MN760663.20 EC HPLC column (analytical), NUCLEODUR PFAS, 3 µm, 50x2 mm Special offer 
Add to Cart
  • Recommended application(s)
  • PFAS
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis + batch document
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR PFAS
  • USP listing
  • Not available or proprietary
  • Surface chemistry
  • Proprietary
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 50 mm
  • Column inner diameter
  • 2 mm
  • Particle type
  • Fully porous particle (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • Not available or proprietary
  • pH stability
  • 1.0–9.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
  • Column volume
  • 0.15 mL
MN760666.20 EC HPLC column (analytical), NUCLEODUR PFAS, 3 µm, 100x2 mm Special offer 
Add to Cart
  • Recommended application(s)
  • PFAS
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis + batch document
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR PFAS
  • USP listing
  • Not available or proprietary
  • Surface chemistry
  • Proprietary
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 100 mm
  • Column inner diameter
  • 2 mm
  • Particle type
  • Fully porous particle (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • Not available or proprietary
  • pH stability
  • 1.0–9.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
  • Column volume
  • 0.3 mL
MN760396.20 EC UHPLC column (analytical), NUCLEODUR C18 Isis, 1.8 µm, 75x2 mm Special offer 
Add to Cart
  • Recommended application(s)
  • (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, RP applications UHPLC, Steroids, Suitable for LC-MS
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC/UHPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
  • No
  • Method
  • HPLC/UHPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR C18 Isis
  • USP listing
  • L1
  • Surface chemistry
  • High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC UHPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 75 mm
  • Column inner diameter
  • 2 mm
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 1.8 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 20.0 %
  • pH stability
  • 1.0–10.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 900 bar
  • Column volume
  • 0.22 mL
MN760397.46 EC HPLC column (analytical), NUCLEODUR C18 Isis, 3 µm, 75x4.6 mm Special offer 
Add to Cart
  • Recommended application(s)
  • (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR C18 Isis
  • USP listing
  • L1
  • Surface chemistry
  • High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 75 mm
  • Column inner diameter
  • 4.6 mm
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 20.0 %
  • pH stability
  • 1.0–10.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
  • Column volume
  • 1.25 mL
MN760400.20 EC HPLC column (analytical), NUCLEODUR C18 Isis, 3 µm, 50x2 mm Special offer 
Add to Cart
  • Recommended application(s)
  • (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR C18 Isis
  • USP listing
  • L1
  • Surface chemistry
  • High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 50 mm
  • Column inner diameter
  • 2 mm
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 20.0 %
  • pH stability
  • 1.0–10.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
  • Column volume
  • 0.15 mL
MN760400.30 EC HPLC column (analytical), NUCLEODUR C18 Isis, 3 µm, 50x3 mm Special offer 
Add to Cart
  • Recommended application(s)
  • (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR C18 Isis
  • USP listing
  • L1
  • Surface chemistry
  • High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 50 mm
  • Column inner diameter
  • 3 mm
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 20.0 %
  • pH stability
  • 1.0–10.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
  • Column volume
  • 0.35 mL
MN760400.40 EC HPLC column (analytical), NUCLEODUR C18 Isis, 3 µm, 50x4 mm Special offer 
Add to Cart
  • Recommended application(s)
  • (o,p,m-) Substituted aromatics, Exceptional steric selectivity, Fat-soluble vitamins, Reversed phase, RP applications HPLC, Steroids, Suitable for LC-MS
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR C18 Isis
  • USP listing
  • L1
  • Surface chemistry
  • High steric selectivity, Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 50 mm
  • Column inner diameter
  • 4 mm
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 3 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 20.0 %
  • pH stability
  • 1.0–10.0
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
  • Column volume
  • 0.62 mL

Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.


Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.


Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.



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