MACHEREY-NAGEL - LC Columns - Chromatography - Lab supplies

Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork. * NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses. * NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications. * Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No.		Product	Net Price
	MN718760	PEEK standard capillary, OD 1/16", 1 m	Special offer	Add to Cart
Recommended application(s) HPLC Storage temperature RT Scope of delivery 1x 1 m PEEK capillary Hazardous material No Capillary inner diameter 0.5 mm, 0.13 mm, 0.17 mm, 0.25 mm, 0.75 mm
	MN719402	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 50-5, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 5 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719403	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 100-5, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 5 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719404	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 500-5, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 5 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719405	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 1000-5, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 5 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719406	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 10000-5, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 5 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719410	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 50-10, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 10 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719411	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 100-10, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 10 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719412	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 500-10, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 10 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL
	MN719413	HPLC column, analytical, PS/DVB polymer, NUCLEOGEL GPC 1000-10, 300x7.7 mm	Special offer	Add to Cart
Recommended application(s) Gel permeation chromatography (GPC), GPC clean-up of samples without water content, Only for water-insoluble substances, Size exclusion chromatography (SEC) Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method Special analytical HPLC Brand NUCLEOGEL Base material Spherical polystyrene – divinylbenzene polymer matrix Phase NUCLEOGEL GPC USP listing Not available or not applicable Surface chemistry Highly crosslinked macroporous, spherical polystyrene – divinylbenzene polymer (PS/DVB) Mode Size exclusion chromatography (SEC) / Gel permeation chromatography (GPC) Type VA HPLC column (analytical) Hardware Stainless steel Column length 300 mm Column inner diameter 7.7 mm Particle type Fully porous particles (FPP), Macroporous Particle shape Spherical Particle size 10 µm Eluent in HPLC column Toluene Endcapped No Carbon content Not available or not applicable pH stability Not available or not applicable Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 150 °C Pressure stability (max) 150 bar Column volume 14 mL

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Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.

Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.

Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.

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