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Agilent offers the largest, most modern liquid chromatography (LC) column portfolios for small molecule analysis, with columns suitable for nearly all separation modes and applications.


Whether you are performing conventional or ultra-fast chromatography, separating biomolecules, or analyzing complex compounds, the industry’s highest-performing compounds deliver fast, reproducible results – all engineered with unparalleled quality and reliability.

Catalog No. Product Net Price
AGIL5190-2527 Agilent Bio SEC-5, 300Å, 7.8 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa. 1362 
Add to Cart
  • Inner Diameter (ID)
  • 7.8 mm
  • Length
  • 150 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 5000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 300 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2528 Agilent Bio SEC-5, 300Å, 4.6 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa. 1567 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 300 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 5000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 300 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2529 Agilent Bio SEC-5, 300Å, 4.6 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa. 1267 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 150 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 5000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 300 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2530 Agilent Bio SEC-5, 300Å, 7.8 x 50 mm, 5 µm, guard. A size exclusion guard column to protect and extend the lifetime of a longer analytical SEC column. 617 
Add to Cart
  • Inner Diameter (ID)
  • 7.8 mm
  • Length
  • 50 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 5000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 300 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2531 Agilent Bio SEC-5, 500Å, 7.8 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa. 1941 
Add to Cart
  • Inner Diameter (ID)
  • 7.8 mm
  • Length
  • 300 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 15000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 500 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2532 Agilent Bio SEC-5, 500Å, 7.8 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa. 1482 
Add to Cart
  • Inner Diameter (ID)
  • 7.8 mm
  • Length
  • 150 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 15000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 500 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2533 Agilent Bio SEC-5, 500Å, 4.6 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa. 1590 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 300 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 15000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 500 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2534 Agilent Bio SEC-5, 500Å, 4.6 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa. 1238 
Add to Cart
  • Inner Diameter (ID)
  • 4.6 mm
  • Length
  • 150 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 15000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 500 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2535 Agilent Bio SEC-5, 500Å, 7.8 x 50 mm, 5 µm, guard. A size exclusion guard column to protect and extend the lifetime of a longer analytical SEC column. 641 
Add to Cart
  • Inner Diameter (ID)
  • 7.8 mm
  • Length
  • 50 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Guard Column
  • Yes
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 15000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 500 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC
AGIL5190-2536 Agilent Bio SEC-5, 1000Å, 7.8 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of large biological molecules such as adeno associated virus and virus-like particles. Resolving range 15 kDa - 5 MDa. 1941 
Add to Cart
  • Inner Diameter (ID)
  • 7.8 mm
  • Length
  • 300 mm
  • Phase
  • SEC
  • UNSPSC Code
  • 41115711
  • Brand
  • Agilent Bio SEC-5
  • Hardware
  • SS
  • LC Platform
  • Stainless Steel
  • Maximum Temperature
  • 80 °C
  • Molecular Weight Lower Limit
  • 50000 Da
  • Part Number Application
  • Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
  • Part Number Application Domain
  • Biopharma
  • Particle Size
  • 5 µm
  • Particle Type
  • Fully Porous
  • pH Range
  • 2-8.5
  • Pore Size
  • 1000 Å
  • Pressure Rating
  • 240 bar
  • Separation Mode
  • Size Exclusion (SEC)
  • Shipping Solvent
  • Phosphate Buffer
  • Technique
  • LC

Overview

The small molecule column portfolio includes state-of-the-art superficially porous InfinityLab Poroshell 120 columns as well as traditional fully porous ZORBAX, Polaris, and Pursuit columns.

When you choose Agilent small molecule LC columns, you get more than just a dependable product. You also get unmatched technical support from the world’s largest chromatography supplier.


Agilent gel permeation chromatography (GPC) and size exclusion chromatography (SEC) columns and standards have been the top-selling in the polymer industry for over 40 years. GPC and SEC is used to determine the molecular weight and size distribution of macromolecules. SEC and GPC columns generate accurate, reliable data for critical polymer parameters, including dispersity (PDI), molecular weight mass average (Mw), number average (Mn), and z-average (Mz).


The importance of understanding the attributes of a biologic drug, and the processes used to create it, cannot be underestimated.  Agilent AdvanceBio LC columns are designed and produced to provide results you can be sure of when analyzing highly complex biotherapeutic molecules and monitoring their purity, potency, and other critical quality attributes.



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