Stable in all aqueous buffers commonly used in Protein A Chromatography
Storage
2 to 8°C, 20% Ethanol
BioProcess resin
Yes
Ligand
Alkali-stabilized Protein A-derived (E. coli)
Matrix
Highly cross-linked agarose
Pressure/Flow Specification
Minimum 250 cm/h. Determined in an AxiChrom 1000 column, bed height 20 cm, and operating pressure < 3 bar. Measured using process buffers with the same viscosity as water at 20°C
pH stability, CIP
2–13.7
pH stability, operational
3–12
Binding Capacity/ml Chromatography Medium
~ 60 mg human IgG/mL resin at 2.4 min residence time
Stable in all aqueous buffers commonly used in Protein A Chromatography
Storage
2 to 8°C, 20% Ethanol
BioProcess resin
Yes
Ligand
Alkali-stabilized Protein A-derived (E. coli)
Matrix
Highly cross-linked agarose
Pressure/Flow Specification
Minimum 250 cm/h. Determined in an AxiChrom 1000 column, bed height 20 cm, and operating pressure < 3 bar. Measured using process buffers with the same viscosity as water at 20°C
pH stability, CIP
2–13.7
pH stability, operational
3–12
Binding Capacity/ml Chromatography Medium
~ 60 mg human IgG/mL resin at 2.4 min residence time
Stable in all aqueous buffers commonly used in Protein A Chromatography
Storage
2 to 8°C, 20% Ethanol
BioProcess resin
Yes
Ligand
Alkali-stabilized Protein A-derived (E. coli)
Matrix
Highly cross-linked agarose
Pressure/Flow Specification
Minimum 250 cm/h. Determined in an AxiChrom 1000 column, bed height 20 cm, and operating pressure < 3 bar. Measured using process buffers with the same viscosity as water at 20°C
pH stability, CIP
2–13.7
pH stability, operational
3–12
Binding Capacity/ml Chromatography Medium
~ 60 mg human IgG/mL resin at 2.4 min residence time
Stable in all aqueous buffers commonly used in Protein A Chromatography
Storage
2 to 8°C, 20% Ethanol
BioProcess resin
Yes
Ligand
Alkali-stabilized Protein A-derived (E. coli)
Matrix
Highly cross-linked agarose
Pressure/Flow Specification
Minimum 250 cm/h. Determined in an AxiChrom 1000 column, bed height 20 cm, and operating pressure < 3 bar. Measured using process buffers with the same viscosity as water at 20°C
pH stability, CIP
2–13.7
pH stability, operational
3–12
Binding Capacity/ml Chromatography Medium
~ 60 mg human IgG/mL resin at 2.4 min residence time
Stable in all aqueous buffers commonly used in Protein A Chromatography
Storage
2 to 8°C, 20% Ethanol
BioProcess resin
Yes
Ligand
Alkali-stabilized Protein A-derived (E. coli)
Matrix
Highly cross-linked agarose
Pressure/Flow Specification
Minimum 250 cm/h. Determined in an AxiChrom 1000 column, bed height 20 cm, and operating pressure < 3 bar. Measured using process buffers with the same viscosity as water at 20°C
pH stability, CIP
2–13.7
pH stability, operational
3–12
Binding Capacity/ml Chromatography Medium
~ 60 mg human IgG/mL resin at 2.4 min residence time
Antibody affinity purification using protein A resins is an effective chromatography technique for purifying recombinant monoclonal antibodies for research and bioprocessing
Affinity resins – specific groups
Affinity resins for purifying specific groups of biomolecules
Affinity resins - tagged proteins
Purify fusion proteins with his-tag, GST, and MBP affinity resins
Desalting and buffer exchange resins
Hydrophobic interaction resins
Resins for hydrophobic interaction chromatography (HIC)
Immunoprecipitation resins
Resins for protein immunoprecipitation using protein A or protein G
Ion exchange resins
Chromatography resins for anion exchange and cation exchange chromatography
Multimodal resins
Resins for mixed mode or multimodal chromatography
Reversed phase resins
Size exclusion resins
Size exclusion resins for separation and desalting
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