0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM β-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).
0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM β-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).
0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM β-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).
For Ni2+-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min
For Ni2+-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min
Antibody affinity purification using protein A resins is an effective chromatography technique for purifying recombinant monoclonal antibodies for research and bioprocessing
Affinity resins – specific groups
Affinity resins for purifying specific groups of biomolecules
Affinity resins - tagged proteins
Purify fusion proteins with his-tag, GST, and MBP affinity resins
Desalting and buffer exchange resins
Hydrophobic interaction resins
Resins for hydrophobic interaction chromatography (HIC)
Immunoprecipitation resins
Resins for protein immunoprecipitation using protein A or protein G
Ion exchange resins
Chromatography resins for anion exchange and cation exchange chromatography
Multimodal resins
Resins for mixed mode or multimodal chromatography
Reversed phase resins
Size exclusion resins
Size exclusion resins for separation and desalting