MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN730971.3T	CHROMABOND QuEChERS Mix II Sticks KIT, Extraction, AOAC 2007.01, 7.5 g	Special offer	Add to Cart
Hazardous material No Method Extraction, AOAC 2007.01 Brand CHROMABOND Phase Mix II Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 50 mL with screw cap Hardware Polypropylene (PP) and coated aluminium foil (Sticks) Column volume 50 mL Recommended application(s) Food samples, Forensic and drug samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730989.3T	CHROMABOND QuEChERS Mix XII Sticks KIT, 7.5 g	Special offer	Add to Cart
Hazardous material No Method Extraction, original Brand CHROMABOND Phase Mix XII Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 50 mL with screw cap Hardware Polypropylene (PP) and coated aluminium foil (Sticks) Column volume 50 mL Recommended application(s) Food samples, Forensic and drug samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730648.3	CHROMABOND QuEChERS Mix XII Sticks, Extraction original, 5 g	Special offer	Add to Cart
Hazardous material No Method Extraction, original Brand CHROMABOND Phase Mix XII Mode Dispersive SPE (dSPE) Hardware Sticks (coated aluminum packets) Recommended application(s) Food samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730970.3T	CHROMABOND QuEChERS Mix I, Extraction, EN 15662, KIT, 6.5 g	Special offer	Add to Cart
Hazardous material No Method Extraction, EN 15662 Brand CHROMABOND Phase Mix I Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 50 mL with screw cap Hardware Polypropylene (PP) and coated aluminium foil (Sticks) Column volume 50 mL Recommended application(s) Food samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730657	CHROMABOND QuEChERS Mix XIX, Clean-up, AOAC 2007.01, 15 mL c. tubes, 0.25 g	Special offer	Add to Cart
Hazardous material No Method Clean-up, AOAC 2007.01 Brand CHROMABOND Phase Mix XIX Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Avocado, Baby food, Beef, Cereals, Chicken, Dairy products, Food samples, Forensic and drug samples, Nuts, Oils, Pesticides, Pork, QuEChERS, Sample preparation, Samples with higher fat content, Soil Storage temperature RT
	MN730984	CHROMABOND QuEChERS Mix XII, Extraction, original, 15 mL c. tubes, 1.25 g	Special offer	Add to Cart
Hazardous material No Method Extraction, original Brand CHROMABOND Phase Mix XII Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Food samples, Forensic and drug samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730779	CHROMABOND QuEChERS Mix M1, special clean-up-mix for mycotoxin analysis	Special offer	Add to Cart
Hazardous material No Method Clean-up, special Brand CHROMABOND Phase Mix M1 Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Mycotoxins from cereals (e.g. wheat, rye) Storage temperature RT
	MN7300008	CHROMABOND QuEChERS Mix L, Clean-up, 15 mL c. Tubes, 1.35 g	Special offer	Add to Cart
Hazardous material No Method Clean-up Brand CHROMABOND Phase Mix L Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) PFAS in food (e.g. milk, curd cheese, quark, bread, brussel sprouts, spinach, eggs), QuEChERS, Sample preparation Storage temperature RT
	MN730011MPS	SPE MPS columns, CHROMABOND C18 ec, 1 mL/100 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), automated Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE MPS column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Tubular syringe-shaped SPE column - already equipped with special cap and cannula to be used in Gerstel MultiPurposeSampler (MPS) Column volume 1 mL Filling quantity 100 mg Column volume/adsorbent weight 1 mL/100 mg Recommended application(s) Aflatoxins, Chloramphenicol, Pesticides, Steroids, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730012MPS	SPE MPS columns, CHROMABOND C18 ec, 3 mL/200 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), automated Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE MPS column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Tubular syringe-shaped SPE column - already equipped with special cap and cannula to be used in Gerstel MultiPurposeSampler (MPS) Column volume 3 mL Filling quantity 200 mg Column volume/adsorbent weight 3 mL/200 mg Recommended application(s) Aflatoxins, Chloramphenicol, Pesticides, Steroids, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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