MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN730970.100	CHROMABOND QuEChERS Mix I Extraction, EN 15662, 15 mL c.tubes, 6.5 g, BIG	Special offer	Add to Cart
Hazardous material No Method Extraction, EN 15662 Brand CHROMABOND Phase Mix I Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Food samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730970.3	CHROMABOND QuEChERS Mix I Sticks, Extraction, EN 15662, 6.5 g	Special offer	Add to Cart
Hazardous material No Method Extraction, EN 15662 Brand CHROMABOND Phase Mix I Mode Dispersive SPE (dSPE) Hardware Sticks (coated aluminum packets) Recommended application(s) Food samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730971	CHROMABOND QuEChERS Mix II, Extraction, AOAC 2007.01, 15 mL c. tubes, 7.5 g	Special offer	Add to Cart
Hazardous material No Method Extraction, AOAC 2007.01 Brand CHROMABOND Phase Mix II Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Food samples, Forensic and drug samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730971.1	CHROMABOND QuEChERS Mix II, Extraction, AOAC 2007.01, 50 mL c.tubes, 7.5 g	Special offer	Add to Cart
Hazardous material No Method Extraction, AOAC 2007.01 Brand CHROMABOND Phase Mix II Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 50 mL with screw cap Hardware Polypropylene (PP) Column volume 50 mL Recommended application(s) Food samples, Forensic and drug samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730971.3	CHROMABOND QuEChERS Mix II Sticks, Extraction, AOAC 2007.01, 7.5 g	Special offer	Add to Cart
Hazardous material No Method Extraction, AOAC 2007.01 Brand CHROMABOND Phase Mix II Mode Dispersive SPE (dSPE) Hardware Sticks (coated aluminum packets) Recommended application(s) Food samples, Forensic and drug samples, Pesticides, QuEChERS, Sample preparation Storage temperature RT
	MN730972	CHROMABOND QuEChERS Mix III, Clean-up, EN 15662, 15 mL c. tubes, 1.05 g	Special offer	Add to Cart
Hazardous material No Method Clean-up, EN 15662 Brand CHROMABOND Phase Mix III Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Apple, Apricot, Asparagus, Broccoli, Food samples, Forensic and drug samples, Pear, Pesticides, Pineapple, QuEChERS, Sample preparation, Samples with low fat content, Strawberry Storage temperature RT
	MN730972.100	CHROMABOND QuEChERS Mix III, Clean-up, EN 15662, 15 mL c. tubes, 1.05 g, BIG	Special offer	Add to Cart
Hazardous material No Method Clean-up, EN 15662 Brand CHROMABOND Phase Mix III Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Apple, Apricot, Asparagus, Broccoli, Food samples, Forensic and drug samples, Pear, Pesticides, Pineapple, QuEChERS, Sample preparation, Samples with low fat content, Strawberry Storage temperature RT
	MN730973	CHROMABOND QuEChERS Mix IV, Clean-up, EN 15662, 15 mL c. tubes, 1.065 g	Special offer	Add to Cart
Hazardous material No Method Clean-up, EN 15662 Brand CHROMABOND Phase Mix IV Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Carrot, Coffee, Food samples, Forensic and drug samples, Lettuce, Pesticides, QuEChERS, Sample preparation, Samples with moderate content of chlorophyll and carotinoids (pigments), Tea Storage temperature RT
	MN730974	CHROMABOND QuEChERS Mix VI, Clean-up, EN 15662, 15 mL c. tubes, 1.2 g	Special offer	Add to Cart
Hazardous material No Method Clean-up, EN 15662 Brand CHROMABOND Phase Mix VI Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Avocado, Baby food, Beef, Cereals, Chicken, Dairy products, Food samples, Forensic and drug samples, Nuts, Oils, Pesticides, Pork, QuEChERS, Sample preparation, Samples with higher fat content, Soil Storage temperature RT
	MN730974.100	CHROMABOND QuEChERS Mix VI, Clean-up, EN 15662, 15 mL c. tubes, 1.2 g, BIG	Special offer	Add to Cart
Hazardous material No Method Clean-up, EN 15662 Brand CHROMABOND Phase Mix VI Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Avocado, Baby food, Beef, Cereals, Chicken, Dairy products, Food samples, Forensic and drug samples, Nuts, Oils, Pesticides, Pork, QuEChERS, Sample preparation, Samples with higher fat content, Soil Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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