MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN731776	SPE cartridges, CHROMAFIX HR-XCW, Large	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMAFIX Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE cartridge Hardware Polypropylene (PP) cartridges with polyethylene (PE) filter elements Column shape Tubular syringe-shaped cartridge with Luer tip inlet and outlet Filling quantity 450 mg Recommended application(s) Active ingredients from strongly matrix contaminated samples (urine, plasma, serum), Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 85 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN731801	SPE cartridges, CHROMAFIX C18, Small	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMAFIX Phase C18 Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18, RP18) modified silica phase, not endcapped Column type SPE cartridge Hardware Polypropylene (PP) cartridges with polyethylene (PE) filter elements Column shape Tubular syringe-shaped cartridge with Luer tip inlet and outlet Filling quantity 90 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, Organic hydrophobic compounds, PAH, PCBs, Pesticides, Preservatives, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN731802	SPE cartridges, CHROMAFIX C18, Medium	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMAFIX Phase C18 Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18, RP18) modified silica phase, not endcapped Column type SPE cartridge Hardware Polypropylene (PP) cartridges with polyethylene (PE) filter elements Column shape Tubular syringe-shaped cartridge with Luer tip inlet and outlet Filling quantity 200 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, Organic hydrophobic compounds, PAH, PCBs, Pesticides, Preservatives, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN731803	SPE cartridges, CHROMAFIX C18, Large	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMAFIX Phase C18 Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18, RP18) modified silica phase, not endcapped Column type SPE cartridge Hardware Polypropylene (PP) cartridges with polyethylene (PE) filter elements Column shape Tubular syringe-shaped cartridge with Luer tip inlet and outlet Filling quantity 560 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, Organic hydrophobic compounds, PAH, PCBs, Pesticides, Preservatives, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN738638	8-well strip sealing caps for PP tube strips (REF 738652)	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), accessory Brand CHROMABOND Hardware Polypropylene (PP) Recommended application(s) Accessories for SPE applications (solid phase extraction - sample preparation Storage temperature RT
	MN730566	Adapter for 30, 70 mL CHROMABOND SPE polypropylene (PP) columns	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), accessory Brand CHROMABOND Storage temperature RT
	MN730101	Adapters for 1, 3, 6 mL CHROMABOND SPE polypropylene (PP) columns	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), accessory Brand CHROMABOND Storage temperature RT
	MN730385	Adapters for 15, 45, 70 mL CHROMABOND SPE polypropylene (PP) columns	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), accessory Brand CHROMABOND Storage temperature RT
	MN730105	Adapters, PVDF, for 3 and 6 mL CHROMABOND SPE glass columns	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), accessory Brand CHROMABOND Storage temperature RT
	MN730155	CHROMABOND QuEChERS Mix VI, Clean-up, EN 15662, 15 mL c.tubes, 0.6 g	Special offer	Add to Cart
Hazardous material No Method Clean-up, EN 15662 Brand CHROMABOND Phase Mix VI Mode Dispersive SPE (dSPE) Column type Centrifuge tube, 15 mL with screw cap Hardware Polypropylene (PP) Column volume 15 mL Recommended application(s) Avocado, Baby food, Beef, Cereals, Chicken, Dairy products, Food samples, Forensic and drug samples, Nuts, Oils, Pesticides, Pork, QuEChERS, Sample preparation, Samples with higher fat content, Soil Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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