MACHEREY-NAGEL - LC Columns - Chromatography - Lab supplies

Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork. * NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses. * NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications. * Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No.		Product	Net Price
	MN760554.40	EC HPLC column (analytical), NUCLEODUR HILIC, 5 µm, 100x4 mm	Special offer	Add to Cart
Recommended application(s) Amino acids, Highly polar analytes, HILIC applications HPLC, Hydrophilic compounds, Nucleosides, Oligonucleotides, Organic polar acids and bases, Peptides, Polar natural compounds, Water-soluble vitamins Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR HILIC USP listing Not available or proprietary Surface chemistry Ammonium – sulfonic acid (zwitterionic) modification Mode Hydrophilic Interaction Chromatography (HILIC) Type EC HPLC column (analytical) Hardware Stainless steel Column length 100 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped No Carbon content 7.0 % pH stability 2.0–8.5 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 1.25 mL
	MN760554.46	EC HPLC column (analytical), NUCLEODUR HILIC, 5 µm, 100x4.6 mm	Special offer	Add to Cart
Recommended application(s) Amino acids, Highly polar analytes, HILIC applications HPLC, Hydrophilic compounds, Nucleosides, Oligonucleotides, Organic polar acids and bases, Peptides, Polar natural compounds, Water-soluble vitamins Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEODUR Base material NUCLEODUR - high purity silica Phase NUCLEODUR HILIC USP listing Not available or proprietary Surface chemistry Ammonium – sulfonic acid (zwitterionic) modification Mode Hydrophilic Interaction Chromatography (HILIC) Type EC HPLC column (analytical) Hardware Stainless steel Column length 100 mm Column inner diameter 4.6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped No Carbon content 7.0 % pH stability 2.0–8.5 Pore size 110 Å Specific surface according to BET 340 m²/g Temperature stability (max) 60 °C Pressure stability (max) 600 bar Column volume 1.65 mL
	MN736596.40	EC HPLC column (analytical), NUCLEOGEN 60-7 DEAE, 7 µm, 125x4 mm	Special offer	Add to Cart
Recommended application(s) HPLC, Oligonucleotides up to chain lengths of 40 bases Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEN Base material Silica gel Phase NUCLEOGEN 60-7 DEAE USP listing Not available or proprietary Surface chemistry Silica gel based anion exchanger with hydrophilic coating Mode Ion exchange (IEX), Special application or diverse Type EC HPLC column (analytical) Hardware Stainless steel Column length 125 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 7 µm Eluent in HPLC column Methanol Endcapped No Carbon content Not available or proprietary pH stability 2.5–8.0 Pore size 60 Å Specific surface according to BET Not available or proprietary Temperature stability (max) 70 °C Pressure stability (max) 250 bar
	MN736597.100	VP HPLC column (preparative), NUCLEOGEN 60-7 DEAE, 7 µm, 125x10 mm	Special offer	Add to Cart
Recommended application(s) HPLC, Oligonucleotides up to chain lengths of 40 bases Storage temperature RT Scope of delivery 1x semi-preparative HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, preparative Brand NUCLEOGEN Base material Silica gel Phase NUCLEOGEN 60-7 DEAE USP listing Not available or proprietary Surface chemistry Silica gel based anion exchanger with hydrophilic coating Mode Ion exchange (IEX), Special application or diverse Type VP HPLC column (preparative) Hardware Stainless steel Column length 125 mm Column inner diameter 10 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 7 µm Eluent in HPLC column Methanol Endcapped No Carbon content Not available or proprietary pH stability 2.5–8.0 Pore size 60 Å Specific surface according to BET Not available or proprietary Temperature stability (max) 70 °C Pressure stability (max) 250 bar
	MN736601	VA HPLC column (analytical), NUCLEOGEN 4000-7 DEAE, 7 µm, 125x6 mm	Special offer	Add to Cart
Recommended application(s) DNA restriction fragments, HPLC, Messenger RNA, Plasmids, Ribosomal RNA, Very high molecular weight nucleic acids (e.g., 1–50 megadaltons), Viral RNA Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEN Base material Silica gel Phase NUCLEOGEN 4000-7 DEAE USP listing Not available or proprietary Surface chemistry Silica gel based anion exchanger with hydrophilic coating Mode Ion exchange (IEX), Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 125 mm Column inner diameter 6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 7 µm Eluent in HPLC column Methanol Endcapped No Carbon content Not available or proprietary pH stability 2.5–8.0 Pore size 4000 Å Specific surface according to BET Not available or proprietary Temperature stability (max) 70 °C Pressure stability (max) 250 bar
	MN736602.100	VP HPLC column (preparative), NUCLEOGEN 4000-7 DEAE, 7 µm, 125x10 mm	Special offer	Add to Cart
Recommended application(s) DNA restriction fragments, HPLC, Messenger RNA, Plasmids, Ribosomal RNA, Very high molecular weight nucleic acids (e.g., 1–50 megadaltons), Viral RNA Storage temperature RT Scope of delivery 1x semi-preparative HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, preparative Brand NUCLEOGEN Base material Silica gel Phase NUCLEOGEN 4000-7 DEAE USP listing Not available or proprietary Surface chemistry Silica gel based anion exchanger with hydrophilic coating Mode Ion exchange (IEX), Special application or diverse Type VP HPLC column (preparative) Hardware Stainless steel Column length 125 mm Column inner diameter 10 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 7 µm Eluent in HPLC column Methanol Endcapped No Carbon content Not available or proprietary pH stability 2.5–8.0 Pore size 4000 Å Specific surface according to BET Not available or proprietary Temperature stability (max) 70 °C Pressure stability (max) 250 bar
	MN736598	VA HPLC column (analytical), NUCLEOGEN 500-7 DEAE, 7 µm, 125x6 mm	Special offer	Add to Cart
Recommended application(s) 5S RNA, HPLC, Intermediate molecular weight range (25,000–1,000,000 daltons), Messenger RNA, tRNA, Viroids Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOGEN Base material Silica gel Phase NUCLEOGEN 500-7 DEAE USP listing Not available or proprietary Surface chemistry Silica gel based anion exchanger with hydrophilic coating Mode Ion exchange (IEX), Special application or diverse Type VA HPLC column (analytical) Hardware Stainless steel Column length 125 mm Column inner diameter 6 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 7 µm Eluent in HPLC column Methanol Endcapped No Carbon content Not available or proprietary pH stability 2.5–8.0 Pore size 500 Å Specific surface according to BET Not available or proprietary Temperature stability (max) 70 °C Pressure stability (max) 250 bar
	MN736599.100	VP HPLC column (preparative), NUCLEOGEN 500-7 DEAE, 7 µm, 125x10 mm	Special offer	Add to Cart
Recommended application(s) 5S RNA, HPLC, Intermediate molecular weight range (25,000–1,000,000 daltons), Messenger RNA, tRNA, Viroids Storage temperature RT Scope of delivery 1x semi-preparative HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, preparative Brand NUCLEOGEN Base material Silica gel Phase NUCLEOGEN 500-7 DEAE USP listing Not available or proprietary Surface chemistry Silica gel based anion exchanger with hydrophilic coating Mode Ion exchange (IEX), Special application or diverse Type VP HPLC column (preparative) Hardware Stainless steel Column length 125 mm Column inner diameter 10 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 7 µm Eluent in HPLC column Methanol Endcapped No Carbon content Not available or proprietary pH stability 2.5–8.0 Pore size 500 Å Specific surface according to BET Not available or proprietary Temperature stability (max) 70 °C Pressure stability (max) 250 bar
	MN720081.40	EC HPLC column (analytical), NUCLEOSIL CHIRAL-1, 5 µm, 250x4 mm	Special offer	Add to Cart
Recommended application(s) Chiral, enantiomers, Enantiomers with two polar functional groups, HPLC, Lactic acid, α-amino acids, α-hydroxycarboxylic acids Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL CHIRAL-1 USP listing L32 Surface chemistry L-hydroxyproline – Cu²⁺ complexes (chiral selector) modification Mode Special application or diverse Type EC HPLC column (analytical) Hardware Stainless steel Column length 250 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column 0.5 mmol/L copper sulfate solution Endcapped No Carbon content Not available or proprietary pH stability 2.0–8.0 Pore size 120 Å Specific surface according to BET 200 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar Column volume 3.15 mL
	MN720088.40	EC HPLC column (analytical), NUCLEOSIL CHIRAL-2, 5 µm, 250x4 mm	Special offer	Add to Cart
Recommended application(s) Chiral, enantiomers, Control in chiral organic syntheses, Diastereomers, Enantiomers, HPLC, Pesticides, Pharmaceuticals, Propionic acid derived herbicides, Stereoisomers Storage temperature RT Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL CHIRAL-2 USP listing Not available or proprietary Surface chemistry N-(3,5-dinitrobenzoyl)-D-phenylglycine (chiral selector) modification Mode Special application or diverse Type EC HPLC column (analytical) Hardware Stainless steel Column length 250 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column n-heptane – 2-propanol – TFAA (trifluoroacetic acid) (100/0.05/0.05, v/v/v) Endcapped No Carbon content Not available or proprietary pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar Column volume 3.15 mL

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Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.

Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.

Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.

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