1x analytical HPLC column, instruction leaflet, certificate of analysis
Hazardous material
No
Method
HPLC, analytical
Brand
NUCLEOGEN
Base material
Silica gel
Phase
NUCLEOGEN 500-7 DEAE
USP listing
Not available or proprietary
Surface chemistry
Silica gel based anion exchanger with hydrophilic coating
Mode
Ion exchange (IEX), Special application or diverse
Type
VA HPLC column (analytical)
Hardware
Stainless steel
Column length
125 mm
Column inner diameter
6 mm
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Particle size
7 µm
Eluent in HPLC column
Methanol
Endcapped
No
Carbon content
Not available or proprietary
pH stability
2.5–8.0
Pore size
500 Å
Specific surface according to BET
Not available or proprietary
Temperature stability (max)
70 °C
Pressure stability (max)
250 bar
DESCRIPTION
Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For the separation of tRNA, 5S RNA, viroids and messenger RNA in the intermediate molecular weight range (25,000–1,000,000 daltons) with recoveries > 95 %. Capacity 730 A260 for a 125 x 6 mm ID column, 1940 A260 for a 125 x 10 mm ID column.