MACHEREY-NAGEL - LC Columns - Chromatography - Lab supplies

Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork. * NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses. * NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications. * Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No.		Product	Net Price
	MN721188.30	EC HPLC guard column (analytical), NUCLEOSIL CHIRAL-1, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL CHIRAL-1 USP listing L32 Surface chemistry L-hydroxyproline – Cu²⁺ complexes (chiral selector) modification Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column 0.5 mmol/L copper sulfate solution Endcapped No Carbon content Not available or proprietary pH stability 2.0–8.0 Pore size 120 Å Specific surface according to BET 200 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721190.30	EC HPLC guard column (analytical), NUCLEOSIL CHIRAL-2/3, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL CHIRAL-2/3 USP listing L36 Surface chemistry Not available or proprietary Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column n-heptane – 2-propanol – TFAA (trifluoroacetic acid) (100/0.05/0.05, v/v/v) Endcapped No Carbon content Not available or proprietary pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721194.30	EC HPLC guard column (analytical), NUCLEOSIL 100-5 C8, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 C8 USP listing L7 Surface chemistry Octyl (C8, RP8, MOS) modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped No Carbon content 8.5 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721196.30	EC HPLC guard column (analytical), NUCLEOSIL 100-3 C18 HD, 3 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-3 C18 HD USP listing L1 Surface chemistry High density monomeric octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particle (FPP) Particle shape Spherical Particle size 3 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 20.0 % pH stability 2.0–9.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721402.30	EC HPLC guard column (analytical), RESOLVOSIL BSA-7, 7 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand RESOLVOSIL Base material NUCLEOSIL - the standard silica Phase RESOLVOSIL BSA-7 USP listing L75 Surface chemistry Bovine serum albumin (BSA, chiral selector) modification Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 7 µm Eluent in HPLC column 0.1 mol/L phosphate buffer, pH 7.5 + 2 % 1-propanol Endcapped No Carbon content Not available or proprietary pH stability 5.0–8.0 Pore size 300 Å Specific surface according to BET 100 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721469.30	EC HPLC guard column (analytical), NUCLEODEX ALPHA-PM, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEODEX Base material NUCLEOSIL - the standard silica Phase NUCLEODEX ALPHA-PM USP listing Not available or proprietary Surface chemistry Permethylated α-cyclodextrin (chiral selector) modification Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column CH₃OH (methanol) – 50 mmol/L phosphate, pH 3 (70/30, v/v) Endcapped No Carbon content Not available or proprietary pH stability 3.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 50 °C Pressure stability (max) 400 bar
	MN721473.30	EC HPLC guard column (analytical), NUCLEOSIL 50-5 C18 ec, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 50-5 C18 ec USP listing L1 Surface chemistry Octadecyl (C18, ODS) modification, Octadecyl phase (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 14.5 % pH stability 2.0–8.0 Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721518.30	EC HPLC guard column (analytical), NUCLEOSIL 100-5, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 USP listing L3 Surface chemistry Unmodified silica gel (SiOH) Mode Normal phase (NP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column n-heptane Endcapped No Carbon content Not available or proprietary pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721649.30	EC HPLC guard column (analytical), NUCLEOSIL 100-3 C18 Nautilus, 3 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-3 C18 Nautilus USP listing L1, L60 Surface chemistry Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 3 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 16.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721916.30	EC HPLC guard column (analytical), NUCLEOSIL 300-5 C4, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 300-5 C4 USP listing L26 Surface chemistry Medium density monomeric butyl (C4) wide-pore modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 2.0 % pH stability 2.0–8.0 Pore size 300 Å Specific surface according to BET 100 m²/g Temperature stability (max) 60 °C Pressure stability (max) 300 bar

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Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.

Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.

Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.

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