MACHEREY-NAGEL - LC Columns - Chromatography - Lab supplies

Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork. * NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses. * NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications. * Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No.		Product	Net Price
	MN719540	VA HPLC guard column (analytical), NUCLEOGEL SCX, 8 µm, 5x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 2x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polymer-based Phase NUCLEOGEL SCX USP listing L22 Surface chemistry Polymer-based strongly acidic cation exchanger SO₃⁻, hydrophilic gel matrix Mode Ion exchange (IEX) Type VA HPLC guard column (analytical) Hardware Stainless steel Column length 5 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8 µm Eluent in HPLC column 0.1 mol/L Na₂SO₄ (sodium sulfate) + 0.02 % NaN₃ (sodium azide) Endcapped No Carbon content Not available or proprietary pH stability 1.0–13.0 Pore size 1000 Å Pressure stability (max) 200 bar
	MN719543	VA HPLC guard column (analytical), NUCLEOGEL Anion I, 10 µm, 21x4 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 2x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polymer-based Phase NUCLEOGEL Anion I USP listing Not available or proprietary Surface chemistry Strongly basic polymer-based anion exchanger Mode Ion exchange (IEX), Special application or diverse Type VA HPLC guard column (analytical) Hardware Stainless steel Column length 21 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 10 µm Eluent in HPLC column 4 mmol/L salicylate buffer, pH 7.8 Endcapped No Carbon content Not available or proprietary pH stability 1.0–13.0 Specific surface according to BET Not available or proprietary Pressure stability (max) 200 bar
	MN719575	CC HPLC guard column (analytical), NUCLEOGEL SUGAR 810 H, 8-10 µm, 30x4 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 2x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Sulfonated spherical PS/DVB resin Phase NUCLEOGEL SUGAR 810 H USP listing L17 Surface chemistry Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), H⁺ form Mode Special application or diverse Type CC HPLC guard column (analytical) Hardware Stainless steel Column length 30 mm Column inner diameter 4 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8–10 µm Eluent in HPLC column 5 mmol/L H₂SO₄ (sulfuric acid) Endcapped No Carbon content Not available or proprietary Pore size 100 Å Temperature stability (max) 90 °C Pressure stability (max) 70 bar
	MN719600	VA HPLC guard column (analytical), NUCLEOGEL SAX, 8 µm, 5x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 2x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOGEL Base material Polymer-based Phase NUCLEOGEL SAX USP listing L23 Surface chemistry Polymer-based strongly basic anion exchanger -N+(CH3)3, gel matrix quaternized PEI Mode Ion exchange (IEX) Type VA HPLC guard column (analytical) Hardware Stainless steel Column length 5 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 8 µm Eluent in HPLC column 0.1 mol/L Na₂SO₄ (sodium sulfate) + 0.02 % NaN₃ (sodium azide) Endcapped No Carbon content Not available or proprietary pH stability 1.0–14.0 Pore size 1000 Å Pressure stability (max) 200 bar
	MN721020.30	EC HPLC guard column (analytical), NUCLEOSIL 100-5 NH2, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 NH2 USP listing L8 Surface chemistry Aminopropyl (NH2) modification Mode Multi-mode (NP and RP), Normal phase (NP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column n-heptane Endcapped No Carbon content 3.5 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721022.30	EC HPLC guard column (analytical), NUCLEOSIL 100-3 C18, 3 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-3 C18 USP listing L1 Surface chemistry Octadecyl (C18, ODS) modification, Octadecyl phase (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 3 µm Eluent in HPLC column Acetonitrile – water Endcapped Yes Carbon content 18.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721024.30	EC HPLC guard column (analytical), NUCLEOSIL 100-5 SA, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 SA USP listing L9 Surface chemistry Sulfonic acid (strong acidic cation exchanger - SCX) modification Mode Ion exchange (IEX) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column 0.15 mol/L (NH₄)₂HPO₄ (diammonium hydrogen phosphate), pH 5 Endcapped No Carbon content 6.5 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721025.30	EC HPLC guard column (analytical), NUCLEOSIL 100-5 SB, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 SB USP listing L14 Surface chemistry Quaternary ammonium (strong basic anion exchanger - SAX) modification Mode Ion exchange (IEX) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column 0.15 mol/L (NH₄)₂HPO₄ (diammonium hydrogen phosphate), pH 5 Endcapped No Carbon content 10.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721030.30	EC HPLC guard column (analytical), NUCLEOSIL 100-7 C2, 7 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-7 C2 USP listing L16 Surface chemistry Dimethyl (C2, RP2) modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 7 µm Eluent in HPLC column Acetonitrile – water Endcapped No Carbon content 3.5 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721039.30	EC HPLC guard column (analytical), NUCLEOSIL 100-5 CN-RP, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 CN-RP USP listing L10 Surface chemistry Cyano, nitrile (CN) modification Mode Multi-mode (NP and RP), Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water Endcapped No Carbon content 5.0 % pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar

<< 121 122 123 124 125126127 128 129 130 131 >>

Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.

Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.

Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.

Resources

Brochure-Nucleodur-Nucleoshell

Catalog-Chromatography-EN

Related Categories