MACHEREY-NAGEL - LC Columns - Chromatography - Lab supplies

Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork. * NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses. * NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications. * Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No.		Product	Net Price
	MN721167.30	EC HPLC guard column (analytical), NUCLEOSIL 50-5, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 50-5 USP listing L3 Surface chemistry Unmodified silica gel (SiOH) Mode Normal phase (NP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column n-heptane Endcapped No Carbon content Not available or proprietary pH stability 2.0–8.0 Pore size 50 Å Specific surface according to BET 420 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721168.20	EC HPLC guard column (analytical), NUCLEOSIL 100-5 C18 PAH, 5 µm, 4x2 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 C18 PAH USP listing L118 Surface chemistry Special octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 2 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water (70/30, v/v) Endcapped Yes Carbon content Not available or proprietary pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721168.30	EC HPLC guard column (analytical), NUCLEOSIL 100-5 C18 PAH, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL 100-5 C18 PAH USP listing L118 Surface chemistry Special octadecyl (C18, ODS) modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water (70/30, v/v) Endcapped Yes Carbon content Not available or proprietary pH stability 2.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721169.30	EC HPLC guard column (analytical), NUCLEOSIL Anion II, 10 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 3x analytical HPLC guard columns Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL Anion II USP listing Not available or proprietary Surface chemistry Strongly basic anion exchanger (SAX) modification Mode Ion exchange (IEX) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 10 µm Eluent in HPLC column 0.15 mol/L (NH₄)₂HPO₄ (diammonium hydrogen phosphate), pH 5 Endcapped No Carbon content Not available or proprietary pH stability 2.0–7.5 Pore size 300 Å Specific surface according to BET 100 m²/g Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721170.30	EC HPLC guard column (analytical), NUCLEOSIL Carbohydrate, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEOSIL Base material NUCLEOSIL - the standard silica Phase NUCLEOSIL Carbohydrate USP listing L8 Surface chemistry Aminopropyl (NH2) modification Mode Reversed phase (RP) Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water (79/21, v/v) Endcapped No Carbon content Not available or proprietary pH stability 2.0–9.0 Specific surface according to BET Not available or proprietary Temperature stability (max) 60 °C Pressure stability (max) 400 bar
	MN721171.30	EC HPLC guard column (analytical), NUCLEODEX BETA-OH, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEODEX Base material NUCLEOSIL - the standard silica Phase NUCLEODEX BETA-OH USP listing L45 Surface chemistry β-cyclodextrin (chiral selector) modification Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column CH₃OH (methanol) – 0.1 % TEAA (triethylammonium acetate), pH 4 (55/45, v/v) Endcapped No Carbon content Not available or proprietary pH stability 3.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 50 °C Pressure stability (max) 400 bar
	MN721176.30	EC HPLC guard column (analytical), NUCLEODEX BETA-PM, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEODEX Base material NUCLEOSIL - the standard silica Phase NUCLEODEX BETA-PM USP listing L45 Surface chemistry Permethylated β-cyclodextrin (chiral selector) modification Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column CH₃OH (methanol) – 0.1 % TEAA (triethylammonium acetate), pH 4 (65/35, v/v) Endcapped No Carbon content Not available or proprietary pH stability 3.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 50 °C Pressure stability (max) 400 bar
	MN721178.30	EC HPLC guard column (analytical), NUCLEODEX GAMMA-PM, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEODEX Base material NUCLEOSIL - the standard silica Phase NUCLEODEX GAMMA-PM USP listing Not available or proprietary Surface chemistry Permethylated γ-cyclodextrin (chiral selector) modification Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column CH₃OH (methanol) – 0.1 % TEAA (triethylammonium acetate), pH 4 (55/45, v/v) Endcapped No Carbon content Not available or proprietary pH stability 3.0–8.0 Pore size 100 Å Specific surface according to BET 350 m²/g Temperature stability (max) 50 °C Pressure stability (max) 400 bar
	MN721185.30	EC HPLC guard column (analytical), NUCLEOCEL DELTA S, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEOCEL Base material Silica gel Phase NUCLEOCEL DELTA S USP listing L40 Surface chemistry Special modification with chiral selector - cellulose tris-(3,5-dimethylphenylcarbamate) Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column n-heptane – 2-propanol (90/10, v/v) Endcapped No Carbon content Not available or proprietary pH stability 1.0–9.0 Specific surface according to BET Not available or proprietary Temperature stability (max) 40 °C Pressure stability (max) 150 bar
	MN721186.30	EC HPLC guard column (analytical), NUCLEOCEL DELTA RP S, 5 µm, 4x3 mm	Special offer	Add to Cart
Recommended application(s) Guard column, ideal protection for your main column, HPLC Storage temperature RT Scope of delivery 1x analytical HPLC guard column Hazardous material No Method HPLC, analytical Brand NUCLEOCEL Base material Silica gel Phase NUCLEOCEL DELTA RP S USP listing L40 Surface chemistry Special modification with chiral selector - cellulose tris-(3,5-dimethylphenylcarbamate) Mode Special application or diverse Type EC HPLC guard column (analytical) Hardware Stainless steel Column length 4 mm Column inner diameter 3 mm Particle type Fully porous particles (FPP) Particle shape Spherical Particle size 5 µm Eluent in HPLC column Acetonitrile – water (40/60, v/v) Endcapped No Carbon content Not available or proprietary pH stability 1.0–9.0 Specific surface according to BET Not available or proprietary Temperature stability (max) 40 °C Pressure stability (max) 150 bar

<< 124 125 126 127 128129130 131 132 133 134 >>

Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.

Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.

Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.

Resources

Brochure-Nucleodur-Nucleoshell

Catalog-Chromatography-EN

Related Categories