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Stainless steel HPLC columns for analytical or preparative applictions from MACHEREY‑NAGEL are a great choice for your daily labwork.

* NUCLEOSIL HPLC phases have gained wide acceptance as routine chromatographic packings for very different fields of modern chromatography. It is one of the first spherical silicas used in HPLC and is an absolutely reliable choice for routine analyses.

* NUCLEODUR as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years. NUCLEODUR HPLC columns with 1.8 µm particle size for increased separation efficiency are used for UHPLC applications.

* Due to core-shell technology NUCLEOSHELL columns provide highly efficient separation results with a comparably lower back pressure to fully porous materials.

Catalog No. Product Net Price
MN763538.20 EC HPLC guard column (analytical), NUCLEOSHELL PFP, 2.7 µm, 4x2 mm Special offer 
Add to Cart
  • Recommended application(s)
  • Guard column, ideal protection for your main column, HPLC
  • Storage temperature
  • RT
  • Scope of delivery
  • 3x analytical HPLC guard columns
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEOSHELL
  • Base material
  • NUCLEOSHELL - core-shell silica
  • Phase
  • NUCLEOSHELL PFP
  • USP listing
  • L43
  • Surface chemistry
  • Pentafluorophenylpropyl (PFP) modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC guard column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 4 mm
  • Column inner diameter
  • 2 mm
  • Particle type
  • Core-shell, superficially porous particles (SPP)
  • Particle shape
  • Spherical
  • Particle size
  • 2.7 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 3.0 %
  • pH stability
  • 1.0–9.0
  • Pore size
  • 90 Å
  • Specific surface according to BET
  • 90 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
MN763538.30 EC HPLC guard column (analytical), NUCLEOSHELL PFP, 2.7 µm, 4x3 mm Special offer 
Add to Cart
  • Recommended application(s)
  • Guard column, ideal protection for your main column, HPLC
  • Storage temperature
  • RT
  • Scope of delivery
  • 3x analytical HPLC guard columns
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEOSHELL
  • Base material
  • NUCLEOSHELL - core-shell silica
  • Phase
  • NUCLEOSHELL PFP
  • USP listing
  • L43
  • Surface chemistry
  • Pentafluorophenylpropyl (PFP) modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC guard column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 4 mm
  • Column inner diameter
  • 3 mm
  • Particle type
  • Core-shell, superficially porous particles (SPP)
  • Particle shape
  • Spherical
  • Particle size
  • 2.7 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 3.0 %
  • pH stability
  • 1.0–9.0
  • Pore size
  • 90 Å
  • Specific surface according to BET
  • 90 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
MN763738.20 EC HPLC guard column (analytical), NUCLEOSHELL Phenyl-Hexyl, 2.7 µm, 4x2 mm Special offer 
Add to Cart
  • Recommended application(s)
  • Guard column, ideal protection for your main column, HPLC
  • Storage temperature
  • RT
  • Scope of delivery
  • 3x analytical HPLC guard columns
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEOSHELL
  • Base material
  • NUCLEOSHELL - core-shell silica
  • Phase
  • NUCLEOSHELL Phenyl-Hexyl
  • USP listing
  • L11
  • Surface chemistry
  • Phenylhexyl modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC guard column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 4 mm
  • Column inner diameter
  • 2 mm
  • Particle type
  • Core-shell, superficially porous particles (SPP)
  • Particle shape
  • Spherical
  • Particle size
  • 2.7 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 4.5 %
  • pH stability
  • 1.0–10.0
  • Pore size
  • 90 Å
  • Specific surface according to BET
  • 130 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
MN763738.30 EC HPLC guard column (analytical), NUCLEOSHELL Phenyl-Hexyl, 2.7 µm, 4x3 mm Special offer 
Add to Cart
  • Recommended application(s)
  • Guard column, ideal protection for your main column, HPLC
  • Storage temperature
  • RT
  • Scope of delivery
  • 3x analytical HPLC guard columns
  • Hazardous material
  • No
  • Method
  • HPLC, analytical
  • Brand
  • NUCLEOSHELL
  • Base material
  • NUCLEOSHELL - core-shell silica
  • Phase
  • NUCLEOSHELL Phenyl-Hexyl
  • USP listing
  • L11
  • Surface chemistry
  • Phenylhexyl modification
  • Mode
  • Reversed phase (RP)
  • Type
  • EC HPLC guard column (analytical)
  • Hardware
  • Stainless steel
  • Column length
  • 4 mm
  • Column inner diameter
  • 3 mm
  • Particle type
  • Core-shell, superficially porous particles (SPP)
  • Particle shape
  • Spherical
  • Particle size
  • 2.7 µm
  • Eluent in HPLC column
  • Acetonitrile – water
  • Endcapped
  • Yes
  • Carbon content
  • 4.5 %
  • pH stability
  • 1.0–10.0
  • Pore size
  • 90 Å
  • Specific surface according to BET
  • 130 m²/g
  • Temperature stability (max)
  • 60 °C
  • Pressure stability (max)
  • 600 bar
MN719539 Guard column holder B for separate use of 5x3 mm Valco-Type HPLC guard columns Special offer 
Add to Cart
  • Guard column dimension
  • 5 mm length, 3 mm ID
  • Accessory for HPLC hardware
  • VA
  • Recommended application(s)
  • HPLC
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x Guard column holder B (incl. nuts and ferrules)
  • Hazardous material
  • No
  • Hardware
  • VA
MN719538 Guard column holder C for separate use of 21x4mm Valco-Type HPLC guard columns Special offer 
Add to Cart
  • Guard column dimension
  • 21 mm length, 4 mm ID
  • Accessory for HPLC hardware
  • VA
  • Recommended application(s)
  • HPLC
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x Guard column holder C
  • Hazardous material
  • No
  • Hardware
  • VA
MN718769 Guillotine cutter for PEEK and PTFE capillaries, red Special offer 
Add to Cart
  • Recommended application(s)
  • HPLC
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x guillotine cutter
  • Hazardous material
  • No
MN706290 Cutter for 1/16" capillaries from steel or polymer materials Special offer 
Add to Cart
  • Recommended application(s)
  • HPLC
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x cutter
  • Hazardous material
  • No
MN713551.0100 HPLC packing material (adsorbents, bulk), NUCLEODUR 100-50 Special offer 
Add to Cart
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x 100 g LC adsorbent in glass container
  • Hazardous material
  • No
  • Method
  • HPLC, packing material (adsorbent, bulk)
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR
  • USP listing
  • L3
  • Surface chemistry
  • Unmodified silica gel (SiOH)
  • Mode
  • Normal phase (NP)
  • Type
  • Fully porous particles (FPP)
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 50 µm
  • Endcapped
  • No
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g
MN713600.0100 HPLC packing material (adsorbents, bulk), NUCLEODUR 100-20 Special offer 
Add to Cart
  • Storage temperature
  • RT
  • Scope of delivery
  • 1x 100 g LC adsorbent in glass container
  • Hazardous material
  • No
  • Method
  • HPLC, packing material (adsorbent, bulk)
  • Brand
  • NUCLEODUR
  • Base material
  • NUCLEODUR - high purity silica
  • Phase
  • NUCLEODUR
  • USP listing
  • L3
  • Surface chemistry
  • Unmodified silica gel (SiOH)
  • Mode
  • Normal phase (NP)
  • Type
  • Fully porous particles (FPP)
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Spherical
  • Particle size
  • 20 µm
  • Endcapped
  • No
  • Pore size
  • 110 Å
  • Specific surface according to BET
  • 340 m²/g

Overview

High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures and their analysis. At the beginning the technique was also called high pressure liquid chromatography due to the high back pressure of the column. HPLC offers qualitative (identification of substances) and quantitative (concentration determination) analysis by comparison with standard substances. The term HPLC was introduced in the 1970s, for the delineation of the high-performance method to the in the 1930s developed column liquid chromatography (column chromatography). At the beginning of the 21st century the HPLC was complemented by the even more efficient UHPLC (ultra high performance liquid chromatography). Hereby even higher pressures (> 400 bar) result in shorter analysis time and enhanced efficiency enabling a higher sample throughput with smaller sample volumes.


Detailed information of the particular properties of the high-purity silica phases NUCLEODUR, the established standard silica NUCLEOSIL and the modern Core-Shell material NUCLEOSHELL as well as phases for special separations can be found at the equivalent HPLC- and UHPLC-columns.


Each MACHEREY‑NAGEL HPLC / UHPLC column is individually tested according to the most important characteristic parameters in quality control and the results are documented in a certificate of analysis.



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