Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents. Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli. Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping. Retained binding capacity of up to 10 mg/mL resin. Simplified sample handling – fewer steps needed compared with conventional workflow. Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification. EDTA-resistant resin for IMAC chromatographyNi Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.His tag purification of large sample volumesThe flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.Successful use in published researchScientists use Ni Sepharose excel to purify his-tagged recombinant proteins from eukaryotic and prokaryotic samples, as cited in these publications:Human transferrin receptor 1 secreted from BHK cellsProprotein convertase subtilisin/kexin type 9 from HEK293 supernatantsMembrane protein insertase EcYidC from E. coli for crystallizationHelicobacter pylori adhesin BabA from periplasmic extracts Convenient prepacked columnsNi Sepharose excel resin is also available in the following formats:HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.