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Thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC), also called planar chromatography are based on a multistage distribution process involving

* Suitable adsorbents (the stationary phase) coated as a thin layer onto a suitable support / backing (e.g., glass plate, polyester or aluminum sheet)

* Solvents or solvent mixtures (the mobile phase or eluent)

* Sample molecules (analytes)

Today TLC has gained increasing importance as an analytical separation technique, which is probably due to effects of instrumentation and automation. At the same time the applicability of thin layer chromatography was enhanced by development of new adsorbents and backings. MACHEREY-NAGEL offers a versatile range of ready-to-use layers, which are the result of 50 years of continuous research and development.


Catalog No. Product Net Price
MN818153 TLC aluminum sheets, cellulose layer, ALUGRAM CEL 300, 20x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC)
  • Brand
  • ALUGRAM
  • Phase
  • CEL 300
  • Mode
  • Normal phase (NP)
  • Base material
  • Aluminum sheets coated with cellulose
  • Surface chemistry
  • Native fibrous cellulose
  • Recommended application(s)
  • Amino acids, Carbohydrates, Carboxylic acids, Polar substances
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–20 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN818155 TLC aluminum sheets, cellulose layer, ALUGRAM CEL 300, 4x8 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC)
  • Brand
  • ALUGRAM
  • Phase
  • CEL 300
  • Mode
  • Normal phase (NP)
  • Base material
  • Aluminum sheets coated with cellulose
  • Surface chemistry
  • Native fibrous cellulose
  • Recommended application(s)
  • Amino acids, Carbohydrates, Carboxylic acids, Polar substances
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–20 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN818156 TLC aluminum sheets, cellulose layer, ALUGRAM CEL 300 UV254, 20x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC)
  • Brand
  • ALUGRAM
  • Phase
  • CEL 300 UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Aluminum sheets coated with cellulose
  • Surface chemistry
  • Native fibrous cellulose
  • Recommended application(s)
  • Amino acids, Carbohydrates, Carboxylic acids, Polar substances
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–20 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN818157 TLC aluminum sheets, cellulose layer, ALUGRAM CEL 300 UV254, 5x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC)
  • Brand
  • ALUGRAM
  • Phase
  • CEL 300 UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Aluminum sheets coated with cellulose
  • Surface chemistry
  • Native fibrous cellulose
  • Recommended application(s)
  • Amino acids, Carbohydrates, Carboxylic acids, Polar substances
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 2–20 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN818412 TLC aluminum sheets, silica gel layer + kieselguhr, ALUGRAM Xtra SILGUR, 10x20cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC)
  • Brand
  • ALUGRAM Xtra
  • Phase
  • Xtra SILGUR
  • Mode
  • Normal phase (NP)
  • Base material
  • Aluminum sheets coated with silica gel and kieselguhr
  • Surface chemistry
  • Concentrating zone (kieselguhr, diatomaceous earth or diatomite), Unmodified silica gel (SiOH)
  • Recommended application(s)
  • Aflatoxins, Alkaloids, Amines, Amino acids, Analgesics, Antibiotics, Hormones, Marihuana compounds, Normal phase (NP), Patulin, Steroids, Sweeteners, Vitamins
  • Particle type
  • Fully porous particles (FPP)
  • Particle size
  • 5–17 µm
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN807021 TLC glass plates Alox N UV254, aluminum oxide, neutral, 5x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC)
  • Brand
  • ALOX
  • Phase
  • Alox N UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Glass plates coated with aluminum oxide
  • Surface chemistry
  • Aluminum oxide, neutral
  • Recommended application(s)
  • Aliphatic and aromatic compounds, Alkaloids, Steroids, Terpenes
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN807023 TLC glass plates Alox N UV254, aluminum oxide, neutral, 20x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC)
  • Brand
  • ALOX
  • Phase
  • Alox N UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Glass plates coated with aluminum oxide
  • Surface chemistry
  • Aluminum oxide, neutral
  • Recommended application(s)
  • Aliphatic and aromatic compounds, Alkaloids, Steroids, Terpenes
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN807033 TLC glass plates Alox N UV254, aluminum oxide, neutral, 20x20 cm Special offer 
Add to Cart
  • Hazardous material
  • No
  • Method
  • Thin layer chromatography (TLC), preparative (PLC)
  • Brand
  • ALOX
  • Phase
  • Alox N UV254
  • Mode
  • Normal phase (NP)
  • Base material
  • Glass plates coated with aluminum oxide
  • Surface chemistry
  • Aluminum oxide, neutral
  • Recommended application(s)
  • Aliphatic and aromatic compounds, Alkaloids, Steroids, Terpenes
  • Particle type
  • Fully porous particles (FPP)
  • Particle shape
  • Irregular
  • pH stability
  • 2.0–8.0
  • Storage temperature
  • RT
MN814201 TLC test mixture for Micro-Set F1, amino acids Special offer 
Add to Cart
  • Method
  • TLC Micro-Set, accessory
  • Storage temperature
  • 4−8 °C
MN814204 TLC test mixture for Micro-Set F1, heavy metal cations Special offer 
Add to Cart
  • Method
  • TLC Micro-Set, accessory
  • Storage temperature
  • 4−8 °C

Overview

The success of thin layer chromatography as a highly efficient microanalytical separation method is based on a large number of advantageous properties:


High sample throughput in a short time

Suitable for screening tests

Pilot procedure for HPLC and Flash chromatography

After separation the analytical information can be stored for a longer period of time (the TLC ready-to-use layer acts as storage medium for data)

Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later date

Rapid and cost-efficient optimization of the separation due to easy change of mobile and stationary phase



Resources