Commonly used aqueous buffers: 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol
Storage
20% ethanol at 4°C to 30°C
BioProcess resin
Yes
Ion Exchanger Type
Strong anion exchanger
Ionic Capacity
95 to 125 µmol Cl-/mL resin
Ligand
-OCH2CH(OH)CH2OCH2CH(OH)CH2N(CH3)3
Matrix
High-flow agarose
Pressure/Flow Specification
0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height
pH stability, CIP
2–14
pH stability, operational
2–12
Estimated shelf life from manufacture date
5 years
Flow velocity
Up to 300 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa)
Commonly used aqueous buffers: 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol
Storage
20% ethanol at 4°C to 30°C
BioProcess resin
Yes
Ion Exchanger Type
Strong anion exchanger
Ionic Capacity
95 to 125 µmol Cl-/mL resin
Ligand
-OCH2CH(OH)CH2OCH2CH(OH)CH2N(CH3)3
Matrix
High-flow agarose
Pressure/Flow Specification
0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height
pH stability, CIP
2–14
pH stability, operational
2–12
Estimated shelf life from manufacture date
5 years
Flow velocity
Up to 300 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa)
Commonly used aqueous buffers: 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol
Storage
20% ethanol at 4°C to 30°C
BioProcess resin
Yes
Ion Exchanger Type
Strong anion exchanger
Ionic Capacity
95 to 125 µmol Cl-/mL resin
Ligand
-OCH2CH(OH)CH2OCH2CH(OH)CH2N(CH3)3
Matrix
High-flow agarose
Pressure/Flow Specification
0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height
pH stability, CIP
2–14
pH stability, operational
2–12
Estimated shelf life from manufacture date
5 years
Flow velocity
Up to 300 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa)
Commonly used aqueous buffers: 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol
Storage
20% ethanol at 4°C to 30°C
BioProcess resin
Yes
Ion Exchanger Type
Strong anion exchanger
Ionic Capacity
95 to 125 µmol Cl-/mL resin
Ligand
-OCH2CH(OH)CH2OCH2CH(OH)CH2N(CH3)3
Matrix
High-flow agarose
Pressure/Flow Specification
0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height
pH stability, CIP
2–14
pH stability, operational
2–12
Estimated shelf life from manufacture date
5 years
Flow velocity
Up to 300 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa)
Antibody affinity purification using protein A resins is an effective chromatography technique for purifying recombinant monoclonal antibodies for research and bioprocessing
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