MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN730607	SPE adsorbents (bulk), CHROMABOND CN	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase CN Mode Normal phase (NP) Base material Silica gel Surface chemistry Cyanopropyl (CN, nitrile) modified silica phase Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT
	MN730652	SPE adsorbents (bulk), CHROMABOND C2	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase C2 Mode Reversed phase (RP) Base material Silica gel Surface chemistry Dimethyl (C2, RP2) modified silica phase, not endcapped Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Storage temperature RT
	MN730622	SPE adsorbents (bulk), CHROMABOND Florisil	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase Florisil Mode Normal phase (NP) Base material Magnesium silicate Surface chemistry Magnesium silicate (MgO – SiOH 15/85) Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 150–250 µm Particle shape Irregular Storage temperature RT
	MN730602	SPE adsorbents (bulk), CHROMABOND C18	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase C18 Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18, RP18) modified silica phase, not endcapped Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT
	MN730601	SPE adsorbents (bulk), CHROMABOND C8	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase C8 Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octyl (MOS, C8, RP8) modified silica phase, not endcapped Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT
	MN730630	SPE adsorbents (bulk), CHROMABOND PSA (SCX)	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase PSA Mode Ion exchange (IEX) Base material Silica gel Surface chemistry Silica gel with propylsulfonic acid (PSA) modification, very strong cation exchanger (SCX) Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT
	MN730606	SPE adsorbents (bulk), CHROMABOND C6H5	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase C6H5 Mode Reversed phase (RP) Base material Silica gel Surface chemistry Phenyl (C6H5) modified silica phase, not endcapped Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT
	MN730605	SPE adsorbents (bulk), CHROMABOND OH (Diol)	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase OH (Diol) Mode Normal phase (NP) Base material Silica gel Surface chemistry Dihydroxypropyl (Diol, OH) modification Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT
	MN730653	SPE adsorbents (bulk), CHROMABOND Diamino (PSA for QuEChERS)	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase Diamino Mode Dispersive SPE (dSPE), Normal phase (NP) Base material Silica gel Surface chemistry Primary and secondary amine functions (PSA) Selling unit 20 g, 100 g Recommended application(s) Clean-up, Enrichment, Packing material, QuEChERS, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT
	MN730629	SPE adsorbents (bulk), CHROMABOND PCA (WCX)	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE), packing material (adsorbent, bulk) Brand CHROMABOND Phase PCA Mode Ion exchange (IEX) Base material Silica gel Surface chemistry Silica gel with propylcarboxylic acid (PCA) modificatIon, weakly acid cation exchanger (WCX) Recommended application(s) Clean-up, Enrichment, Packing material, Sample preparation, Solid phase extraction, SPE applications Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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