MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN730012	SPE columns, CHROMABOND C18 ec, 45 µm, 3 mL/200 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 200 mg Column volume/adsorbent weight 3 mL/200 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730013	SPE columns, CHROMABOND C18 ec, 45 µm, 3 mL/500 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 500 mg Column volume/adsorbent weight 3 mL/500 mg Selling unit 50 columns, 250 columns Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730014	SPE columns, CHROMABOND C18 ec, 45 µm, 6 mL/500 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 500 mg Column volume/adsorbent weight 6 mL/500 mg Selling unit 30 columns, 250 columns Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730015	SPE columns, CHROMABOND C18 ec, 45 µm, 6 mL/1000 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 1000 mg Column volume/adsorbent weight 6 mL/1000 mg Selling unit 30 columns, 250 columns Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730141	SPE columns, CHROMABOND C18 ec, 45 µm, 6 mL/2000 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 2000 mg Column volume/adsorbent weight 6 mL/2000 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730259	SPE columns, CHROMABOND C18 ec, 45 µm, 70 mL/10000 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 70 mL Filling quantity 10000 mg Column volume/adsorbent weight 70 mL/10000 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730404	SPE columns, CHROMABOND C18 ec, 45 µm, 15 mL/2000 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 15 mL Filling quantity 2000 mg Column volume/adsorbent weight 15 mL/2000 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730405	SPE columns, CHROMABOND C18 ec, 45 µm, 45 mL/5000 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 45 mL Filling quantity 5000 mg Column volume/adsorbent weight 45 mL/5000 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730012G	SPE glass columns, CHROMABOND C18 ec, 45 µm, 3 mL/200 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE glass column Hardware Glass columns (borosilicate type I) with glass fiber (GF) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 200 mg Column volume/adsorbent weight 3 mL/200 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Glass fiber (GF) Storage temperature RT
	MN730014G	SPE glass columns, CHROMABOND C18 ec, 45 µm, 6 mL/500 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 ec Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18 ec, RP18 ec) modified silica phase, endcapped Column type SPE glass column Hardware Glass columns (borosilicate type I) with glass fiber (GF) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 500 mg Column volume/adsorbent weight 6 mL/500 mg Recommended application(s) Aflatoxins, Amphetamines, Antibiotics, Antiepileptics, Barbiturates, Caffeine, Drugs, Fatty acids, Heavy metals, Nicotine, Nonpolar samples, PAH, PCBs, Pesticides, Preservatives, Very well suited for desalting of samples, Vitamins Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Glass fiber (GF) Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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