MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN732960	CHROMABOND Flash BT 4 SiOH cartridges, 40–63 µm, 4 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash BT Column length 10.6 cm Column inner diameter 12.4 mm Column connection Female Luer lock inlet and male Luer lock outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 4 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Removable Typical flow rate 10–50 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 20x CHROMABOND Flash BT cartridges Storage temperature RT
	MN732961	CHROMABOND Flash BT 15 SiOH cartridges, 40–63 µm, 15 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash BT Column length 12.5 cm Column inner diameter 21.2 mm Column connection Female Luer lock inlet and male Luer lock outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 15 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Removable Typical flow rate 25–70 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 20x CHROMABOND Flash BT cartridges Storage temperature RT
	MN732962	CHROMABOND Flash BT 25 SiOH cartridges, 40–63 µm, 25 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash BT Column length 17.3 cm Column inner diameter 21.2 mm Column connection Female Luer lock inlet and male Luer lock outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 25 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Removable Typical flow rate 25–70 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 15x CHROMABOND Flash BT cartridges Storage temperature RT
	MN732963	CHROMABOND Flash BT 40 SiOH cartridges, 40–63 µm, 40 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash BT Column length 17.6 cm Column inner diameter 26.7 mm Column connection Female Luer lock inlet and male Luer lock outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 40 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Removable Typical flow rate 35–80 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 15x CHROMABOND Flash BT cartridges Storage temperature RT
	MN732964	CHROMABOND Flash BT 80 SiOH cartridges, 40–63 µm, 80 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash BT Column length 24.9 cm Column inner diameter 30.9 mm Column connection Female Luer lock inlet and male Luer lock outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 80 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Removable Typical flow rate 45–105 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 12x CHROMABOND Flash BT cartridges Storage temperature RT
	MN732965	CHROMABOND Flash BT 120 SiOH cartridges, 40–63 µm, 120 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash BT Column length 26.2 cm Column inner diameter 37.2 mm Column connection Female Luer lock inlet and male Luer lock outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 120 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Removable Typical flow rate 55–155 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 10x CHROMABOND Flash BT cartridges Storage temperature RT
	MN732966	CHROMABOND Flash BT 200 SiOH cartridges, 40–63 µm, 200 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash BT Column length 21.6 cm Column inner diameter 59.4 mm Column connection Female Luer lock inlet and male Luer lock outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 200 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Removable Typical flow rate 75–225 mL/min pH stability 2.0–8.0 Pressure stability (max) 11 bar Filter element material Polyethylene (PE) Scope of delivery 6x CHROMABOND Flash BT cartridges Storage temperature RT
	MN732967	CHROMABOND Flash BT 330 SiOH cartridges, 40–63 µm, 330 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash BT Column length 28.0 cm Column inner diameter 59.8 mm Column connection Female Luer lock inlet and male Luer lock outlet Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 330 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Removable Typical flow rate 75–225 mL/min pH stability 2.0–8.0 Pressure stability (max) 11 bar Filter element material Polyethylene (PE) Scope of delivery 4x CHROMABOND Flash BT cartridges Storage temperature RT
	MN727423	Flash sea sand, acid washed, preignited	Special offer	Add to Cart
Hazardous material No Method Flash chromatography, accessory Brand CHROMABOND Recommended application(s) Accessories for Flash chromatography (efficient liquid column chromatographic method for preparative applications in the gram range) Scope of delivery 1x 1000 g Flash sea sand Storage temperature RT
	MN730076	SPE columns, CHROMABOND SA, 45 µm, 1 mL/100 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase SA Mode Ion exchange (IEX) Base material Silica gel Surface chemistry Silica gel with benzenesulfonic acid modification, strongly acidic cation exchanger (SCX) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 1 mL Filling quantity 100 mg Column volume/adsorbent weight 1 mL/100 mg Recommended application(s) Amines, Amino acids, Chlorophyll, PCBs Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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