Ion-pairing chromatography, Reversed phase, RP applications HPLC
Storage temperature
RT
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Hazardous material
No
Method
HPLC, analytical
Brand
NUCLEOSIL
Base material
NUCLEOSIL - the standard silica
Phase
NUCLEOSIL 120-5 C4
USP listing
L26
Surface chemistry
Medium density monomeric butyl (C4) modification
Mode
Reversed phase (RP)
Type
EC HPLC column (analytical)
Hardware
Stainless steel
Column length
250 mm
Column inner diameter
4.6 mm
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Particle size
5 µm
Eluent in HPLC column
Acetonitrile – water
Endcapped
Yes
Carbon content
4.0 %
pH stability
2.0–8.0
Pore size
120 Å
Specific surface according to BET
200 m²/g
Temperature stability (max)
60 °C
Pressure stability (max)
400 bar
Column volume
4.15 mL
DESCRIPTION
This HPLC column is packed with butyl modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured exclusively in Germany and is still one of the most used HPLC silicas in quality control around the globe.