DESCRIPTION
For predictable high resolution separation of mono-, di-, and some oligosaccharides, use the Thermo Scientific™ Dionex™ CarboPac™ PA1 (1 mm) columns, when operating in Dual EGC mode on Thermo Scientific™ Dionex™ ICS-6000 HPIC™ systems equipped with Thermo Scientific™ Chromeleon™ CDS software. Dual EGC mode enables electrolytic generation of potassium methane sulfonate and potassium hydroxide gradients (KMSA/KOH) gradient eluent for carbohydrate analysis using a Dionex KOH EGC 400 KOH Eluent Generator Cartridge and a Dionex MSA EGC 400 MSA Eluent Generator Cartridge in series. Using the Dual EGC allows for an expanded set of samples for gradient separations of some oligosaccharides along with mono-, di-, and trisaccharide's using electrolytically generated potassium methane sulfonate and potassium hydroxide gradients with a combined concentration up to 200 mM. Many separations require only an isocratic separation. However, some groups of analytes will require a step or gradient elution. Retention of carbohydrates can be varied with eluent concentration, in some cases changing the elution order as the eluent concentration increases.
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