DESCRIPTION
The Mouse IgG Type and Titer kit is a multiplexed, competition formatted assay to identify and quantify mouse IgG1, IgG2a, IgG2b, and IgG3. It is a no wash, no sample dilution assay that measures both cells and beads and provides these significant advantages over traditional methods:
IgG isotype identification: Guides the decision-making process by identifying monoclonal wells, thus reducing the number of cell sub-cloning steps while facilitating isotype-specific DNA primer sets rather than degenerate primers for cloning or sequencing of antibody variable regions.
Precise total and isotype specific IgG quantification: Downstream confirmatory or functional assays benefit from normalizing the IgG concentration for all clones.
Simultaneous measurement of cell count and cell viability: Identifies the cells healthy enough for the RNA extraction needed for PCR-mediated antibody cloning.
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