DESCRIPTION
The Human General Immune Cell Mediated Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:
Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye.
Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
NK cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3- CD56+ NK cells.
The panel also includes 2 different NK cell functional activation markers, CD69 and CD25.
Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.
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