DESCRIPTION
The Human General Immune Cell Mediated Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:
Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye and mitochondrial membrane potential dye.
Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
Simultaneous analysis of multiple mechanisms of immune cell mediated cell killing in a single, high content assay including mitochondrial membrane depolarization, loss of cell membrane integrity, cell count, and pro-apoptotic protease Granzyme B.
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