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Catalog No. CYT17012001
Part No. 17012001
Supplier Cytiva
Sepharose 4B
Net Price
832
Availability

4 weeks

PROPERTIES
  • Pack size
    1 L
  • Storage
    4 to 30°C, 20% Ethanol
  • Certificate of Analysis
    Yes
  • Matrix
    agarose, 4%, spherical
  • Particle Size
    45 μm-165 μm
  • Pressure/Flow Specification
    70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.
  • pH stability, CIP
    4–9
  • pH stability, operational
    4–9
  • Flow velocity
    < 11.5 cm/h
DESCRIPTION
Sepharose 4B is a well-proven agarose size exclusion chromatography base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 4% Agarose size exclusion chromatography resin. Proven base matrix for coupling affinity ligands Sepharose is a spherical agarose-based size exclusion chromatography matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight Sepharose CL resins are cross-linked derivatives of Sepharose 2B, Sepharose 4B and Sepharose 6B. The cross-linked form of Sepharose is chemically and physically more resistant than Sepharose itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose gels are resistant to organic solvents and are thus the choice for separations in organic solvents
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