rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale. For monoclonal and polyclonal antibody purification at both laboratory and process scale. Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes. Free from animal-derived components. Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool. Fulfills industrial demands for security of supply, robust performance, and regulatory support. Monoclonal and polyclonal antibody purificationrProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity. Protein A resin characteristicsThe recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.Streamlined scale upScale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.Successful use in published researchCytiva's protein A resins are used in a variety of applications, including: Molecular characterization of an arthritogenic collagen peptide interacting with I-ArCompatible empty columns – research scaleCytiva's solutions include a variety of empty columns for packing. rProtein A Sepharose resin is compatible for use with HiScale, Tricorn, and XK columns.Compatible empty columns – bioprocess scaleFor process-scale applications, Cytiva recommends AxiChrom columns. rProtein A Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.Convenient prepacked columns – research scalerProtein A Sepharose Fast Flow is also available in convenient prepacked formats: rProtein A GraviTrap, for use with gravity flowHiTrap rProtein A FF, for use with a syringe, pump, or chromatography systemConvenient prepacked columns – bioprocess scalePrepacked ReadyToProcess columns are available on request, eliminating the need for column packing and cleaning validation.