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Catalog No. CYT17020001
Part No. 17020001
Supplier Cytiva
QAE Sephadex A-50, 100 g
Net Price
618
Availability

4 weeks

PROPERTIES
  • Chromatography technique
    Ion Exchange Chromatography
  • Pack size
    100 g
  • Autoclavable
    In 0.1 M sodium chloride at 121°C for 30 min.
  • Chemical stability
    Stable to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride.
  • Storage
    4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
  • Certificate of Analysis
    Yes
  • BioProcess resin
    Yes
  • Ion Exchanger Type
    Strong anion
  • Ionic Capacity
    2.6–3.4 mmol Cl- /g dry powder
  • Ligand
    Diethyl-(2-hydroxy-propyl)aminoethyl
  • Matrix
    Cross-linked dextran
  • pH stability, CIP
    2–12
  • pH stability, operational
    2–12
  • Avoid Using
    Oxidising agents and dextranase
  • Binding Capacity/ml Chromatography Medium
    thyroglobulin (Mr 669 000)- 1.2 mg/ml drained resin), HSA (Mr 68 000)- 80 mg/ml drained resin), α-lactalbumin (Mr 14 300)- 30 mg/ml drained resin)
DESCRIPTION
QAE Sephadex A-50 is a strong anion exchanger based on the well documented and well proven Sephadex base matrix. Strong anion exchanger suitable batch techniques. High binding capacities. Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages. Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available.
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