DESCRIPTION
GST Detection Module is used for identification of GST fusion proteins using either a biochemical or immunological assay.
GST Detection Module enables sensitive detection of GSTtagged proteins and contains components
For detection using either a biochemical assay where glutathione and 1-chloro-2-4-dititrobenzene (CDNB) serve as substrates for GST to yield a yellow product detectable at 340 nm, or an immunoassay.
GST Detection Module contains components sufficient for 50 detection reactions using both assays, and an instruction booklet.
In the biochemical assay, glutathione and 1-chloro-2-4-dinitrobenzene (CDNB) serve as substrates for GST to yield a yellow product detectable at 340 nm (1). An affinity-purified goat anti-GST polyclonal antibody suitable for Western blots is used in the immunoassay. The GST Detection Module contains sufficient components for 50 detection reactions using either assay: 1-chloro-2-4-dinitrobenzene, Glutathione, Anti-GST Antibody (see Anti-GST Antibody), and instruction booklet.
References1. Habig, W. H. et al. J. Biol. Chem. 249, 7130 (1974).
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