CYT17513203 - Cytiva - Resins - Chromatography - Lab supplies

: Catalog No. CYT17513203

Part No. 17513203
Supplier Cytiva

Glutathione Sepharose 4 Fast Flow, 500 mL

Net Price

10056

Availability

4 weeks

PROPERTIES

Chromatography technique

GST-tagged protein purification
Pack size

25 mL
Chemical stability

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature
Storage

4 to 30°C, 20% Ethanol
Ligand

Glutathione and 10-carbon linker arm
Matrix

Highly cross-linked 4% agarose
Pressure/Flow Specification

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
pH stability, CIP

3–12
pH stability, operational

3–12
Binding Capacity/ml Chromatography Medium

>10 mg/mL
Flow velocity

< 450 cm/h
Ligand Coupling Method

Epoxy activation

DESCRIPTION

Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed. Fast, one-step GST-tagged protein purification. Mild elution conditions preserve protein antigenicity and function. Excellent scale-up due to high flow properties. Resin for GST-tagged protein purificationGlutathione Sepharose 4 Fast Flow is used to purify glutathione S-transferase (GST)-tagged proteins from bacterial lysates. It is also suitable for purifying other S-transferases or glutathione-dependent proteins. Successful use in published researchGlutathione Sepharose 4 Fast Flow resin is used in a variety of applications, including: Characterization of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolasesApical sorting of lysoGPI-anchored proteins independent ofassociation with detergent-resistant membranesStructure and function of the Rad9-binding region of theDNA-damage checkpoint adaptor TopBP1Compatible empty columnsLife Sciences solutions from Cytiva include a variety of empty columns for packing your own columns. Glutathione Sepharose 4 Fast Flow is compatible for use with gravity, HiScale, Tricorn, and XK columns.Convenient prepacked columnsGlutathione Sepharose 4 Fast Flow resin is also available in the following prepacked formats: GSTrap FF: HiTrap format for convenient use with a syringe, a pomp, or a chromatography systemGSTPrep FF: for convenient scale-upOther glutathione agarose products from CytivaLooking for other products to purify GST-tagged proteins? Browse our loose resin and prepacked options or let Cytiva help you find the right GST resin or prepacked format for your needs.