CYT17070905 - Cytiva - Resins - Chromatography - Lab supplies

: Catalog No. CYT17070905

Part No. 17070905
Supplier Cytiva

DEAE Sepharose Fast Flow, 10 L

Net Price

Special offer

Availability

4 weeks

PROPERTIES

Pack size

500 mL
Chemical stability

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage

4 to 30°C, 20% Ethanol
Certificate of Analysis

Yes
BioProcess resin

Yes
Ion Exchanger Type

Weak anion exchanger
Ionic Capacity

0.12–0.15 mmol (Cl^-)/mL resin
Ligand

Diethylaminoethyl
Matrix

cross-linked agarose, 6%, spherical
Pressure/Flow Specification

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
pH stability, CIP

2–14
pH stability, operational

2–12

DESCRIPTION

DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification. Well-proven weak anion exchanger developed for industrial downstream processes. Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals. High chemical stability allows for well proven CIP and sanitization protocols. Hydrophilic base matrix ensures low levels of non-specific binding, and low levels of host cell-derived impurities in the elution pool. Fulfills industrial demands for security of supply, robust performance, and regulatory support. Streamlined scale-upThis resin is part of the Sepharose Fast Flow ion exchange platform, which is well established in industrial downstream processing. Composed of crosslinked 6% agarose beads with diethylaminoethyl (DEAE) weak anion exchange groups, the resin has high chemical stability, allowing well-proven cleaning-in-place (CIP) and sanitization protocols. The highly crosslinked agarose resin enables high flow rates and high productivity. Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, and PreDictor RoboColumns™, and HiTrap columns.Successful use – research scaleDEAE Sepharose Fast Flow resin is used in a variety of research applications, including: Separation ofPI 3-Kinases from guinea pig neutrophilsPurification of myosin phosphatase from chicken smooth muscle Compatible empty columns – research scaleCytiva's solutions include a variety of empty columns for research scale. This DEAE resin is compatible with two of these lines, Tricorn and XK columns.Compatible empty columns – bioprocess scaleFor process-scale applications for this DEAE resin, Cytiva recommends AxiChrom columns. DEAE Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.Prepacked columns – research scaleThis weak anion exchange resin is available in the following formats: HiScreen DEAE FF for scale up and process developmentHiPrep DEAE FF columns for convenient scale-upPrepacked columns – bioprocess scalePrepacked ReadyToProcess columns for bioprocess applications are available on request.