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Catalog No. CYT17070001
Part No. 17070001
Supplier Cytiva
2'5' ADP Sepharose 4B
Net Price
Special offer
Availability

4 weeks

PROPERTIES
  • Pack size
    5 g
  • Chemical stability
    Stable to commonly used aqueous buffers and additives like detergents.
  • Storage
    2 to 8°C
  • Matrix
    Agarose, 4%
  • Particle Size
    45 µm-165 µm
  • pH stability, CIP
    4–10
  • pH stability, operational
    4–10
  • Avoid Using
    High concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents
  • Flow velocity
    < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
  • Ligand Coupling Method
    Cyanogen bromide activation
DESCRIPTION
2’5’ ADP Sepharose 4B resin with immobilized NADP structural analog is primarily used for purification of enzymes requiring NADP as a cofactor. 2'5' ADP is an NADP structural analog immobilized on Sepharose 4B. It binds and immobilizes enzymes requiring this cofactor 2’5’ ADP Sepharose 4B interacts strongly with NADP+-dependent dehydrogenases. Selective elution with gradients of NAD+ or NADP+ has allowed the resolution of complex mixtures of dehydrogenase isoenzymes using 2’5’ ADP Sepharose 4B.
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