These PreDictor™ RoboColumn™ minicolumns for high-throughput process development (HTPD) are prepacked with Ni Sepharose™ excel affinity resin. For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). Supports HTPD — enables parallel screening of chromatographic conditions using a miniaturized column format and robotic workstation. Miniaturized column format — facilitates screening of a wide range of parameters with small sample consumption. Reduced experimental time — automation and parallel screening minimize labor and save time. Increased process understanding — high-throughput workflow allows investigation of enlarged experimental workspace for better process understanding. Packed with Ni Sepharose™ excel resin — designed for his-tagged protein purification with minimized Ni-leakage and maximized protein recovery when samples contain stripping agents. Ni Sepharose™ excel IMAC resin Ni Sepharose™ excel resin is designed for purification of his-tagged proteins when nickel leakage is an issue. Minimize Ni-leakage and maximize protein recovery in his-tag protein purification, even when samples contain nickel stripping agents. The resin flow properties make it suitable for purifications in a wide range of scales. You can load large sample volumes, enabling purification of target proteins at low concentrations. Learn more about Ni Sepharose™ excel IMAC resin. PreDictor™ RoboColumn™ units for HTPD PreDictor™ RoboColumn™ units are prepacked, miniaturized columns that support HTPD using a robotic liquid handling workstation for fully automated and parallel chromatographic separations. HTPD work can be performed using PreDictor™ RoboColumn™ units alone, or as a complement to PreDictor™ 96-well filter plates. PreDictor™ RoboColumn™ units can be operated in true chromatography mode, enabling dynamic binding capacity studies, while 96-well plates enable the study of static binding capacities. PreDictor™ RoboColumn™ units are supplied in rows of eight columns with the same chromatography resin, and can be individually and easily arranged on an array plate containing 96 positions. Read more about HTPD. * RoboColumn is a trademark of Repligen GmbH.