DESCRIPTION
The Glutathione S-transferase (GST) Gene Fusion System from Cytiva is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli.
Enables the site-specific cleavage of fusion proteins containing an accessible Factor Xa protease recognition sequence.
Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow.
Factor Xa, purified from bovine plasma, is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for factor Xa (pGEX-3X, pGEX-5X-1, pGEX-5X-2, and pGEX-5X-3). It specifically cleaves following the tetrapeptide Ile-Glu-Gly-Arg.
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