CYT29148722 - Cytiva - LC Columns - Chromatography - Lab supplies

: Catalog No. CYT29148722

Part No. 29148722
Supplier Cytiva

Superdex™ 75 Increase 5/150 GL

Net Price

1993

Availability

4 weeks

PROPERTIES

Bed volume

~ 24 mL
Bed height

300 to 310 mm
Bed support

Filter
Material (bed support)

Polyethylene (PE) filter
Material (column tube)

Borosilicate glass
Column i.d.

10
Chemical stability

Conditions where the resin may be operated without significant change in function:
• All commonly used aqueous buffers, pH 3 to 12
• Urea, up to 8 M
• Ionic and nonionic detergents, e.g., 1% SDS
• Guanidine hydrochloride, up to 6 M
• Acetic acid, up to 1 M
• Methanol, up to 10%
• Isopropanol, up to 5%
• Dithiothreitol (DTT), up to 5 mM
• Sodium hydroxide, up to 0.5 M
Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function:
• Acetonitrile, up to 30%
• Sodium hydroxide, up to 1 M
• Ethanol, up to 70%
• Methanol, up to 70%
• Isopropanol, up to 30%
• Hydrochloric acid, up to 0.1 M
• Trifluoroacetic acid, up to 1%
• Formic acid, up to 70%
Avoid:
• Oxidizing agents
• Unfiltered samples and eluents
Sample volume

25 to 500 µL
Bed dimensions

10 × 300 mm
Matrix

Composite of cross-linked agarose and dextran
pH stability, operational

3 to 12
Theoretical plates

> 43 000 m^-1

DESCRIPTION

Superdex™ 75 Increase 10/300 GL, 5/150 GL, and 3.2/300 are prepacked columns for versatile use in size exclusion chromatography applications. The columns are intended for use in preparative purification as well as for characterization and analysis of proteins with molecular weights between Mr 3000 and 70 000, such as recombinant tagged proteins. Up to 50% higher resolution compared with predecessor Superdex™ 75, for improved purity and analysis results. Runtime reduced down to one third compared with predecessor Superdex™ 75, with maintained resolution for results faster. Versatile use in both preparative and analytical applications, especially with recombinant tagged proteins. Higher lot-to-lot consistency compared with predecessor Superdex™ 75. Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover. Higher resolution and/or reduced runtime compared with predecessor Superdex™ 75 Increase belongs to the new generation of agarose based SEC media, comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity for improved performance compared with its predecessor Superdex™ 75. Clean in place for versatile use Superdex™ 75 Increase resin is alkali-resistant. Hence, the column can be cleaned in place under alkali conditions to allow use of the same column for different proteins with minimal risk for carryover between samples. The alkali resistance is what differentiates agarose-based resins from silica-based resins. The possibility to clean the resin under harsh alkali conditions also ensures a long column lifespan.