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Catalog No. CYT28985256
Part No. 28985256
Supplier Cytiva
rProtein A Protein G GraviTrap
Net Price
914
Availability

4 weeks

PROPERTIES
  • Format
    Prepacked column
  • Chromatography technique
    Antibody affinity chromatography
  • Bed volume
    1
  • Order Information
    Includes one pack of 10 x1 ml columns
  • Application
    Fast and efficient manual purification of monoclonal and polyclonal antibodies
  • Ligand
    Protein A
  • Scale
    Lab
  • Resin Type
    Sepharose Fast Flow
  • Includes
    1 pack of 10 x 1 ml columns
DESCRIPTION
rProtein A Protein G GraviTrap contains ten columns with 1 mL 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow resins. For general-purpose purification of monoclonal and polyclonal antibodies. Suitable when the affinity for protein A and protein G is not known. GraviTrap prepacked gravity flow columns enable simple one-step purification of proteins in 30 min without the need for equipment. High purity: > 95% in one step. High protein recovery (typically 70% to 80%) due to high binding capacity of ~ 35 mg IgG/mL resin. Fast and simple antibody purificationThis column can be used for manual purification of polyclonal and monoclonal antibodies from cell culture supernatant and biological fluids. It is particularly suitable when the affinity for protein A and protein G is not known. The gravity flow column relies on gravity and is reusable up to five times, so no additional instruments are needed for this protocol. Other Cytiva products for antibody affinity chromatographyCytiva's solutions include a variety of resins, prepacked columns, and other formats for purifying antibodies and antibody fragments.
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