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Catalog No. CYT28903135
Part No. 28903135
Supplier Cytiva
Protein G HP MultiTrap
Net Price
3237
Availability

4 weeks

PROPERTIES
  • Format
    Prepacked 96-well plate
  • Pack size
    4
  • Wetted Part
    No
  • Application
    Centrifugation
  • Storage
    2 -8°C, 20% Ethanol
  • Ligand
    Protein G
  • Chromatography resin
    Protein G Sepharose High Performance
  • Resin suspensions in total volumes
    50 μl
  • Centrifugation force recommended
    <700 x g
  • Guidance
    Prepacked 96-well plates, packed with Protein G Sepharose High Performance, for \ncoupling of an affinity molecule, large sample series or automation.\n\nInstruction supplied with the well-plates. \n\nCollection plates have to be ordered separately\n\nFor smaller scale please use Protein G HP SpinTrap and for larger scale HiTrap \nProtein G HP
  • Operating temp. max.
    30°C
  • Operating temp. min.
    4°C
  • Scale
    Lab
  • Resin Type
    Sepharose High Performance
DESCRIPTION
Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use. Flexible format, protocol for classical elution as well as cross-linking method. Formatted for elution for electrophoresis or LC-MS analysis workflows. Fast binding kinetics and high capacity provides high yield. Easy scale-up with HiTrap Protein G HP prepacked columns. Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses. MultiTrap 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems. Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol) A protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min is also provided. No pretreatment of the sample is required
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