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Catalog No. CYT28903132
Part No. 28903132
Supplier Cytiva
Protein A HP SpinTrap
Net Price
309
Availability

4 weeks

PROPERTIES
  • For use with
    Standard microcentrifuge.
  • Wetted Part
    No
  • Application
    Enrichment of target proteins \nPurification of antibodies
  • Binding Capacity/Column
    > 1 mg human IgG well
  • Sample volume
    600 μl
  • Storage
    2 - 30°C, 20% Ethanol
DESCRIPTION
Protein A HP SpinTrap are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain Protein A Sepharose HP For simple, small-scale antibody purification or enrichment of specific proteins in prepacked, single-use spin columns that are ready to use. Classic and cross-link protocols provide flexibility. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Fast binding kinetics and high capacity provide high yield. Prepacked* with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses. Easy scale-up with HiTrap Protein A HP prepacked columns. Protein A Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required. There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
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